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Graphene sensor based method for detecting MicroRNA

A graphene and sensor technology, applied in the field of biology, can solve problems such as time-consuming, complicated operation, and high requirements for the reaction system, and achieve the effects of simple preparation and operation, high sensitivity, and good stability

Inactive Publication Date: 2015-11-04
SHANGHAI INTEGRATED CIRCUIT RES & DEV CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The existing two types of technologies involve complex operations in the amplification or labeling process, take a long time, and have high requirements for the reaction system.

Method used

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  • Graphene sensor based method for detecting MicroRNA
  • Graphene sensor based method for detecting MicroRNA
  • Graphene sensor based method for detecting MicroRNA

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Embodiment Construction

[0042] In order to make the content of the present invention clearer and easier to understand, the content of the present invention will be further described below in conjunction with the accompanying drawings. Of course, the present invention is not limited to this specific embodiment, and general replacements known to those skilled in the art are also covered within the protection scope of the present invention.

[0043] The following is attached Figure 1-3 The method for detecting MicroRNA based on the graphene sensor of the present invention will be further described in detail with specific examples. in, figure 1 A schematic flow diagram of a method for detecting MicroRNA based on a graphene sensor of a preferred embodiment of the present invention, figure 2 A schematic flow sheet of the graphene modification process for a preferred embodiment of the present invention, image 3 It is a schematic flow chart of the detection process of the MicroRNA to be tested by using...

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Abstract

The present invention provides a graphene sensor based method for detecting MicroRNA. The method comprises the steps of: preparing a graphene field-effect transistor with graphene as channel, and using the transistor as a graphene sensor; in accordance with the MicroRNA sequence to be tested, conducting surface modification of graphene, and combining the DNA probes capable of combining with the MicroRNA to be measured to the functional groups on the surface of the graphene; blocking the remaining functional groups, unbounded with the DNA probes, on the graphene surface; detecting the MicroRNA to be measured by using the modified graphene field sensor, and detecting the MicroRNA to be measured by the difference of the current signals before and after combination of the MicroRNA to be measured with DNA probes on the graphene. The method does not need labeling to achieve detection of MicroRNA and has the advantages of high detection sensitivity, simple preparation and operation, good stability and fast response.

Description

technical field [0001] The invention relates to the technical field of biology, in particular to a method for detecting MicroRNA based on a graphene sensor. Background technique [0002] MicroRNA (microRNAs, miRNA) is a kind of highly conserved single-stranded non-coding RNA with a length of about 17-25nt. miRNA degrades or inhibits mRNA by binding to the 3'untranslated region (3'UTR) or coding region (CDS) of mRNA, and mainly regulates gene expression at the post-transcriptional level. Since the first microRNA was revealed in 1993, more than 30,000 miRNAs have been discovered. Many studies have shown that miRNAs are involved in different physiological and pathological processes, such as cell differentiation, proliferation and apoptosis, and tumorigenesis. Up to 30% of human genes are regulated by miRNA, and abnormal miRNA content can cause cardiovascular disease, cancer and other diseases. For a long time, researchers have studied miRNA from two aspects: regulatory factor...

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Application Information

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IPC IPC(8): G01N27/327
Inventor 佘斯懿胡少坚陈寿面
Owner SHANGHAI INTEGRATED CIRCUIT RES & DEV CENT