Bifunctionalgraphenedielectrophoresis (DEP)-graphenefield effecttransistor (GFET) devices are provided. Aspects of the devices include: a graphenedielectrophoresis (DEP) component and a graphene field effecttransistor (GFET) component, wherein the graphene DEP and GFET components are independently operable. Also provided are methods of evaluating a sample for the presence of an analyte, e.g., by introducing the sample into a bifunctional graphene dielectrophoresis (DEP)-graphene field effecttransistor (GFET) device and obtaining a result from the device providing information as to whether the analyte is present in the sample. In addition, kits comprising the devices or systems described herein are provided. The devises, systems, methods and kits find use in a variety of different applications, including research and diagnostic applications.