A preparation for reducing wrinkles and its preparation method
A preparation and wrinkle technology, applied in the field of beauty, can solve the problems of poor wrinkle removal effect and low efficiency of PRP wrinkle removal, and achieve good wrinkle removal effect, delay skin aging, and promote cell metabolism
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[0049] The invention provides a preparation method of a preparation for reducing wrinkles, comprising:
[0050] Platelet-rich plasma, sodium hyaluronate, and stem cell growth factor are mixed to obtain a wrinkle-reducing formulation.
[0051] In the present invention, the type, source and dosage of the platelet-rich plasma, sodium hyaluronate and stem cell growth factor are consistent with the type, source and dosage of the platelet-rich plasma, sodium hyaluronate and stem cell growth factor described in the technical solution , which will not be repeated here.
[0052] In a preferred embodiment of the present invention, platelet rich plasma, sodium hyaluronate gel and stem cell growth factor solution are mixed to obtain a wrinkle reducing preparation. In the present invention, the type, source and dosage of the platelet-rich plasma, sodium hyaluronate gel and stem cell growth factor solution are the same as those of the platelet-rich plasma, sodium hyaluronate gel and stem c...
Embodiment 1
[0065] Transfer 50mL of peripheral blood to a 50mL centrifuge tube, and centrifuge at 800g relative centrifugal force for 15min; after centrifugation, use a Pasteur pipette to suck out the upper layer of the centrifuged product to obtain plasma;
[0066] Transfer the plasma to a new 15mL centrifuge tube, centrifuge at 800g for 10min, and further remove the red blood cells in the plasma. After the centrifugation, suck out the supernatant of the obtained centrifuged product with a Pasteur pipette to obtain the intermediate product ;
[0067] The intermediate product was transferred to another new 15mL centrifuge tube, and centrifuged at 3000g relative centrifugal force for 5min. After the centrifugation was completed, the supernatant in the centrifuged product was removed with a Pasteur pipette, and 2mL of the lower sediment was retained. Blow off the cells in the lower sediment with a Pasteur pipette to resuspend, transfer the obtained resuspended product to an EP tube, which i...
Embodiment 2
[0072] Transfer 50mL of peripheral blood to a 50mL centrifuge tube, and centrifuge at 800g relative centrifugal force for 15min; after centrifugation, use a Pasteur pipette to suck out the upper layer of the centrifuged product to obtain plasma;
[0073] Transfer the plasma to a new 15mL centrifuge tube, centrifuge at 800g for 10min, and further remove the red blood cells in the plasma. After the centrifugation, suck out the supernatant of the obtained centrifuged product with a Pasteur pipette to obtain the intermediate product ;
[0074]Transfer the intermediate product to another new 15mL centrifuge tube, and centrifuge for 5min with a relative centrifugal force of 3000g. After the centrifugation, remove the supernatant in the centrifuged product with a Pasteur pipette, leave 3mL of the lower sediment, and use Blow off the cells in the lower sediment with a Pasteur pipette to resuspend, transfer the obtained resuspended product to an EP tube, which is platelet-rich plasma (...
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