Effect of olive polyphenol on improving deterioration of aging-related movement function

[0016] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0017] The 2.5-month-old male C57BL/6J mice were randomly divided into two groups A and B, and the 15.5-month-old male C57BL/6J mice were randomly divided into C and D groups. Among them, groups B and D were used as experimental groups, and were given olive polyphenol (HT-Ac aqueous solution) by gavage; groups A and C were used as control groups, and were given a corresponding volume of water. The body weight and food intake of the experimental mice were monitored weekly for 20 weeks. After 20 weeks of HT-Ac intervention, the mice were subjected to Rotarod performance test. The specific plans are shown in the following table:

[0018] group

[0019] 1. Whether olive polyphenol (HT-Ac) has any effect on the body weight of experimental mice.

[0020] Mice were weighed before the start of the experiment (week 0) and at the end of each week and the data were recorded to obtain as follows: figure 1 As shown, the results showed that olive polyphenols (HT-Ac) had no significant effect on the body weight of both young and old mice.

[0021] 2. Olive polyphenols (HT-Ac) can significantly improve the motor coordination function of aged experimental mice.

[0022] The four groups A, B, C, and D were tested by the rotarod behavioral experiment. At the beginning of the experiment, the rotarod instrument was set to the acceleration mode (4rpm-40rpm, 5min, and the experimental cut-off time was set to 6min). The mouse was placed on the rotarod, and the acceleration switch was turned on to start timing. After the mouse fell from the rotarod, the duration of the mouse on the rotarod was recorded. Each mouse was subjected to two experiments, with a 10-minute interval between experiments. The test results obtained are as figure 2 shown, figure 2 It showed that the duration of the rotarod test was significantly shorter than that of group A and group C. Group C: 74±21s VSA group: 166±61s, P﹤0.001). After the polyphenolic component HT-Ac was administered to the aged mice, the duration of the rod test in the D group was significantly increased compared with the C group (C group: 74±21s VSD group: 131±30s, P﹤ 0.05). Interestingly, there was no significant difference between group D and group A (older intervention group: 131±30s vs. group A: 166±61s, P=0.114>0.05).

[0023] Experiments show that compared with young mice, the motor coordination ability and neuromuscular function of old mice do have a serious decline. Intervention with polyphenolic component HT-Ac in old mice can significantly improve the motor coordination ability of old mice. , neuromuscular function.

[0024] The above experiments found that olive polyphenols (HT-Ac) can significantly improve the motor coordination ability of naturally aging mice, suggesting that the experiment proves that polyphenols may be a potential effective drug that can improve the decline of aging-related motor coordination function.