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Amino acid sequence for detecting lung cancer marker MYC epitope and application

A technology of antigenic epitopes and markers, applied in the biological field, can solve problems such as low sensitivity and poor specificity, and achieve high sensitivity and high specificity effects

Active Publication Date: 2015-11-11
JILIN JINUO BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently reported autoantibody detection methods have low sensitivity and poor specificity, and the false negative rate can be as high as 50%.

Method used

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  • Amino acid sequence for detecting lung cancer marker MYC epitope and application
  • Amino acid sequence for detecting lung cancer marker MYC epitope and application
  • Amino acid sequence for detecting lung cancer marker MYC epitope and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Institutional preparation

[0026] 1 The preparation of reagents see tab.2 ~ 7.

[0027]

[0028]

[0029]

[0030]

[0031]

[0032]

[0033] 2 operation

[0034] (1) Bag quilt: The enzyme standard plate was cleaned 3 times, and the working antigen was diluted to the working concentration.

[0035] (2) Add glutamic acid: Wash the buffer 3 times, dilute glutamic acid with a bag to the concentration of 100 μg / ml, 200 μL per hole, 37 ° C or room temperature for 1h;

[0036](3) Add plasma and quality control control (one anti -resistance): The enzyme standard plate is cleaned 3 times, and the plasma is diluted to the appropriate concentration by using the liquid to be diluted by the liquid.37 ° C or room temperature incubation for 1h;

[0037] (4) Two anti -incubation: washing the buffer to clean 3 times, diluted the two -resistance standard solution IGG with the bag, 1: 20000, 100 μL per hole, 37 ° C or room temperature incubation for 1h;

[0038] (5) color rend...

Embodiment 2

[0041] MYC IGG antibody detection of lung cancer patients

[0042] 1 Sample collection: This study selected 225 cases of lung cancer samples from the third hospital of Jilin University and provincial oncology hospitals to diagnose lung cancer samples, of which 124 were lung adenocarcinoma and 101 cases of lung squamous carcinoma.All serum samples have been treated with any anti -cancer treatment before collecting and have comprehensive clinical data and information.At the same time, 198 cases of health control group samples were recruited.Clinical interviews are eliminated with possibility of lung cancer.The health group and the lung cancer group are comparable in gender and age, which is comparable ( P > 0.05)

[0043] 2 Detection results: Myc's own antibody expression level (TAB.8): There are statistical differences compared with the health control group (compared to the health control group ( z = -2.269, P <0.05).

[0044] ROC curve analysis: The IGG antibody detection of lung ...

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Abstract

The invention relates to an amino acid sequence for detecting a lung cancer marker MYC epitope and application, belongs to the technical field of immunology, and the invention provides the amino acid sequence of an antigen of the lung cancer related gene MYC. A MYC polypeptide antigen is used for detecting a corresponding specific autoantibody in the blood of a patient suffering from a lung cancer, the autoantibody can be used as the lung cancer marker to evaluate the risk of occurrence of the lung cancer and prognostic effect. The antigenic polypeptide and an antibody thereof can be used for preparing a predict reagent for early diagnosis and prediction prognosis of the lung cancer and can be used for developing a target drug for treatment of the lung cancer.

Description

Technical field [0001] The present invention is a biotechnology field, and it involves a specifically involved a model with a detection of lung cancer logo MYC polypeptide fragments that detect lung cancer markers. Background technique [0002] Lung cancer is the main tumor of the chest, and its incidence and mortality ranks first among my country's malignant tumors.Due to the lack of specific diagnostic signs in clinical practice, most patients were late when they found that they had the opportunity to cure surgery.Therefore, early detection, early diagnosis, and early treatment are still urgent for lung cancer.A large number of studies have shown that tumor -related antigens in serum or plasma can induce the body to produce their own antibodies. There are tumor antigens in serum patients in tumor patients, as well as their own antibodies against the tumor antigen.Therefore, antibodies can be used to detect tumor antigens, and antigen can be used to detect their own antibodies o...

Claims

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Application Information

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IPC IPC(8): C07K14/82G01N33/68G01N33/574
CPCG01N33/57423G01N33/6884C07K14/82G01N2333/82
Inventor 王雷刘洋
Owner JILIN JINUO BIOLOGICAL ENG CO LTD
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