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Sequence of standard gene of DNA (deoxyribonucleic acid) barcode of aedes and application thereof

A standard gene and barcode technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problem that there is no related gene sequence of Aedes Iriomote, and achieve the effect of shortening identification time, reducing errors, and rapid identification

Inactive Publication Date: 2015-11-11
珠海国际旅行卫生保健中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of this method in the molecular identification of mosquitoes is limited to a few species of mosquitoes, and there are only COI gene sequences of a few species of mosquitoes in Genbank, but there is no related gene sequence of Aedes Iriomote.

Method used

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  • Sequence of standard gene of DNA (deoxyribonucleic acid) barcode of aedes and application thereof
  • Sequence of standard gene of DNA (deoxyribonucleic acid) barcode of aedes and application thereof
  • Sequence of standard gene of DNA (deoxyribonucleic acid) barcode of aedes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] [Example 1 Determination of Aedes Iriomote DNA Barcode Standard Detection Gene]

[0020] 1. Collection and preservation of Aedes Iriomote specimens

[0021] Aedes Iriomote was collected, and the collected specimens were killed by freezing and stored in 75% alcohol directly.

[0022] 2. Pretreatment of test samples

[0023] Take out the Aedes Iriomote specimens preserved in 75% alcohol, and cut off the head, genitals and wings with a dissecting needle, which is convenient for making slices for species identification. The remaining part was transferred into PCR tubes, stored in 75% alcohol, one for each tube, and uniquely numbered for DNA extraction. If long-term storage is required, the PCR tube containing the remaining tissue parts of Aedes Iriomote should be stored in a -20°C refrigerator.

[0024] 3. DNA template preparation

[0025] Mosquito DNA was extracted using a commercially available genomic DNA extraction kit.

[0026] 4. Primer synthesis

[0027] The pr...

Embodiment 2

[0040] [Example 2 Identification of Unknown Mosquitoes Using Barcode Standards to Detect Gene Sequences]

[0041] 1. Collection and preservation of unknown mosquito specimens

[0042] Unknown mosquitoes were collected, and the collected specimens were directly preserved in 75% alcohol after being killed by freezing.

[0043] 2. Pretreatment of test samples

[0044] Take out the unknown mosquito specimen preserved in 75% alcohol, and cut off the head, genital part and wings with a dissecting needle, which is convenient for making slices for species identification. The remaining part was transferred into PCR tubes, stored in 75% alcohol, one for each tube, and uniquely numbered for DNA extraction.

[0045] 3. DNA template preparation

[0046] Mosquito DNA was extracted using a commercially available genomic DNA extraction kit.

[0047] 4. Primer synthesis

[0048] The primers used in this example are as follows:

[0049] Forward primer: TAAACTTCAGGGTGACCAAAAAAATCA

[0050...

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Abstract

The invention discloses a sequence of a standard gene of a DNA (deoxyribonucleic acid) barcode of aedes. The sequence is characterized in that the gene is a CO I (cytochrome oxidase subunit) gene of aedes (verrallina) iriomotensis; the sequence of the gene is shown in SEQ ID NO.1 in a sequence table. The invention also discloses an application of the sequence of the standard gene of the DNA (deoxyribonucleic acid) barcode of aedes to detection and identification of aedes (verrallina) iriomotensis. The sequence provided by the invention is beneficial to achieving fast identification of aedes (verrallina) iriomotensis and shortening the identification time. An identification method fills in gaps in the field of molecular identification of aedes (verrallina) iriomotensis, is more efficient and convenient than traditional morphological identification methods and has the effects of reducing errors and ensuring that the results have reliability.

Description

technical field [0001] The invention relates to the field of species identification, in particular to a standard gene sequence of an Aedes mosquito DNA barcode. Background technique [0002] Aedes (Verrallina) iriomotensis (Tanaka and Mizusawa, 1973), belongs to Diptera, Culicidae, Aedes, and Verrallina. Aedes is the largest genus in the mosquito family, with nearly 1,000 species, and there are more than 100 species in China. Aedes mosquitoes can transmit many diseases and bring harm to human health. Yellow fever and dengue fever are mainly transmitted by Aedes mosquitoes. Aedes aegypti and Aedes albopictus are the most studied mosquitoes in China. However, there are relatively few studies on Aedes Iriomote, and little is known about whether it may carry viruses and transmit diseases, and further research is needed. [0003] The identification and identification of mosquitoes is the basis for monitoring and controlling mosquito-borne infectious diseases, and it is also an...

Claims

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Application Information

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IPC IPC(8): C12N15/53C12Q1/68
Inventor 廉国胜柯明剑林加燕汪海波田绿波莫秋华杨泽冯子力苏影李艳兰
Owner 珠海国际旅行卫生保健中心
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