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Thin-layer chromatographic identification method for inonotus sanghuang

A thin-layer chromatography and identification method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of wrong use of Phellinus linteus, damage to research and development of food, etc.

Inactive Publication Date: 2015-11-11
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of using Phellinus Phellinus, if different kinds of Phellinus Phellinus are misused or abused, this will damage the next research and development of food

Method used

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  • Thin-layer chromatographic identification method for inonotus sanghuang
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  • Thin-layer chromatographic identification method for inonotus sanghuang

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Identification of Phellinus Phellinus by Thin Layer Chromatography

[0026] Accurately weigh 2 g of the powder with a weighing balance, add 50 mL of absolute ethanol, heat to reflux, filter after 3 hours, then evaporate the filtrate to dryness, add 2 mL of methanol to the residue to fully dissolve the Phellinus residue, extract twice with ethyl acetate, 30mL each time. Evaporate the extract to dryness, add methanol to dissolve it, and use this as a sample. According to the test of thin-layer chromatography (Appendix VIB of "Chinese Pharmacopoeia" 2010 Edition), draw 1, 3, and 5 μL of the above solutions and spot them on the same thin plate, with the same developing agent, first develop, then take out, then dry, and place Check under ultraviolet light (365nm), and finally take pictures. The influence of different sample volumes on thin-layer chromatography was investigated, and the results showed that although the sample volumes of 1 μL and 3 μL could run cle...

Embodiment 2

[0028] Investigate the influence of reflux extraction of 100% ethanol and 70% ethanol solution on thin-layer chromatography and the stability of color development, other steps are the same as in Example 1. The result is as figure 2 shown. It can be seen from the figure that the thin layer of the extract with 100% ethanol is better. The application of 70% ethanol reflux to extract the products of the two samples will cause the loss of some products in the sample, so the method of 100% ethanol reflux extraction is used to process the samples.

Embodiment 3

[0030] Investigating the influence of thin plates made of different materials on thin-layer chromatography is the same in other respects, which are carried out in silica gel G plates and polyamide thin plates respectively, and other steps are the same as in Example 1. see Figure 4 , 5 . As can be seen from the figure, Figure 5 The samples in the silica gel G plate in can show obvious bands, while Figure 4 The components of the polyamide thin plate samples cannot be separated well, so the experiment chooses the silica gel G plate as the experimental plate.

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Abstract

The invention discloses a thin-layer chromatographic identification method for inonotus sanghuang. The method particularly comprises the steps that the inonotus sanghuang is ground into powder firstly, 2 g of the powder is accurately weighed through a weighing balance and added into 50 mL ethyl alcohol, after heating reflux is conducted for 3 h, filtration is conducted, then filtrate is evaporated to dryness, 2 mL methyl alcohol is added into residues to make the inonotus sanghuang residues fully dissolved, a test sample solution dissolved with the residues is extracted for two times through 30 mL ethyl acetate, the extraction liquid is evaporated to dryness, the methyl alcohol is added to make the residues dissolved, and the solution serves as a sample for testing; 5 microliters of the sample solution is sucked and dipped on a same thin plate, a same developing agent is adopted, unfolding, taking out and drying in the air are conducted sequentially, the sample is placed under an ultraviolet lamp for inspection at 365 nm wavelength, and photographing is conducted; a clear strip can be stably displayed on the thin plate, regular consistency is presented in the strip of the sample, the fluorescent color development within three hours is stable, and the substance is judged to be the inonotus sanghuang. The fluorescent color development within three hours is stable, the method is simple, efficient and capable of being used for rapidly identifying the inonotus sanghuang.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a thin-layer chromatographic identification method for Phellinus. Background technique [0002] Sang Huang (Inonotus Sang Huang), also known as Sang Er, Sang Chen, is named after parasitic on mulberry trees. As a precious medicinal fungus, Phellinus has the functions of improving the body's immunity, anti-tumor, resisting cell mutation, anti-bacterial, inhibiting liver fibrosis, stopping bleeding, promoting blood circulation and lowering blood sugar, inhibiting bacteria and reducing inflammation, and treating cancer and hepatitis. A large number of scientific studies have found that Phellinus has the effect of inhibiting the growth of cancer cells, and the anti-cancer rate is as high as 96.7%. It is currently internationally recognized as the most effective medicinal fungus in the field of biological anti-cancer. Phellinus is known as "forest gold" because of its extremely high heal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
Inventor 闻燕张攀杰季娟徐安捷李晓彤
Owner JIANGSU UNIV OF SCI & TECH