Label-free okadaic acid sensor based on self-catalytic growth of gold nanoparticles

A technology of okadaic acid and sensors, which is applied in the field of unlabeled okadaic acid sensors, can solve the problems of poor accuracy and repeatability, high maintenance cost, and large instrument size, and achieve simple operation, easy mass production, cost reduction effect

Inactive Publication Date: 2017-10-03
ZHEJIANG UNIV
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Problems solved by technology

[0003] Regarding the detection of OA, there are mainly biological analysis methods, mainly the mouse method, which have the problems of low sensitivity, poor accuracy and repeatability, and poor specificity; chemical instrument analysis methods, such as gas chromatography, liquid chromatography, and liquid-mass spectrometry ( LC-MS), etc., although the detection characteristics are good, but the instrument is large and the maintenance cost is high

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  • Label-free okadaic acid sensor based on self-catalytic growth of gold nanoparticles
  • Label-free okadaic acid sensor based on self-catalytic growth of gold nanoparticles
  • Label-free okadaic acid sensor based on self-catalytic growth of gold nanoparticles

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Embodiment Construction

[0025] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0026] Such as figure 1As shown, the present invention is based on the label-free okadaic acid sensor of nano-gold self-catalytic growth, and the nano-gold particle 10 and the OA aptamer 11 are modified in the gap between the interdigitated electrode pairs of the sensor, and the OA toxin molecule and the OA aptamer The combination of 11 causes structural changes of the OA aptamer 11, exposing the active sites of the gold nanoparticles 10, and the concentration of OA can be calculated from the conductance signal of the sensor after the self-catalytic growth of the gold nanoparticles 10. The okadaic acid sensor of the present invention adopts a label-free detection method, which includes: a silicon dioxide substrate 1, a first comb-toothed electrode 2, a second comb-toothed electrode 3, a first lead 4, a second lead 5, The first pad 6,...

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Abstract

The invention discloses a label-free okadaic acid sensor based on the self-catalytic growth of nano-gold. The okadaic acid (OA) sensor has a silicon dioxide substrate and a pair of comb-shaped interdigitated microelectrodes, and there are gold nanoparticles and OA aptamers in the electrode gaps. Amino groups are modified in the gaps of microelectrodes by silanization; gold nanoparticles are electrostatically bound to amino groups and fixed in the gaps of microelectrodes; OA aptamers can be electrostatically combined with gold nanoparticles and modified in gaps of microelectrodes to complete the OA sensor preparation. OA molecules can specifically bind to OA aptamers, resulting in structural changes of OA aptamers and exposing the active sites of gold nanoparticles. In the mixed solution of glucose and chloroauric acid, the active sites of gold nanoparticles grow self-catalyzed, which causes the change of the conductance signal at both ends of the interdigitated microelectrodes, thereby indirectly measuring the concentration of OA. The invention can be used to realize rapid and label-free detection of OA.

Description

technical field [0001] The invention relates to the technical field of okadaic acid sensor detection, in particular to a label-free okadaic acid sensor based on the self-catalytic growth of nano gold. Background technique [0002] Okadaic acid (OA) is a metabolite of marine algae. During the filter feeding or feeding process of shellfish, fish and other animals, it is transferred by food to their stomach or esophagus, digested, absorbed and Lead to the accumulation of OA in shellfish. OA is a fatty acid derivative of polycyclic polyether, which is the main pathogenic factor of diarrhea shellfish poisoning, and the toxin is still relatively stable to general heating and cooking. It has been confirmed that OA has strong tumor-promoting and carcinogenic effects. Therefore, it is particularly important to test them from a hygiene point of view. [0003] Regarding the detection of OA, there are mainly biological analysis methods, mainly the mouse method, which have the problem...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48
Inventor 苏凯麒王平潘宇祥甘颖王君邹玲王琴方佳如
Owner ZHEJIANG UNIV
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