Method for extracting Anoectochilus roxburghii polysaccharides

An extraction method and polysaccharide extraction technology are applied in the field of extraction of clematis polysaccharide, which can solve the problems of low polysaccharide extraction rate and the like, and achieve the effects of high polysaccharide yield, mild conditions and stable process.

Inactive Publication Date: 2015-11-25
SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0022] The purpose of the present invention is to solve the problem that the extraction rate of polysaccharides is too low at present, and to provide a method for extracting polysaccharides from Aurantia chinensis by hot water extraction

Method used

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  • Method for extracting Anoectochilus roxburghii polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Take the whole clematis herb, wash it, drain the water in a cool and ventilated place, dry it at 50°C, and pass it through a 40-mesh sieve to obtain clematis powder.

[0042] (2) Accurately weigh a certain amount of clematis whole herb powder 50.00g, soak in 10 times the volume (v:w) of 95% ethanol for 12 hours, filter, repeat the treatment twice, combine the two filtrates to concentrate for later use; place the filter residue in a cool place Ethanol was evaporated in a ventilated place, and then dried in an oven at 50°C to obtain a clematis pretreatment powder.

[0043] (3) Accurately weigh 15.00 g of Clematis clematis pretreated powder, add distilled water at a liquid-to-solid ratio of 1:30 (g:mL), bathe in water at 70°C for 90 minutes, filter with suction, and extract repeatedly 4 times. Combine 4 filtrate polysaccharide extracts.

[0044](4) The polysaccharide extract is fixed to volume, and the total sugar and reducing sugar content in the extract are determin...

Embodiment 2

[0046] (1) Take the whole clematis herb, wash it, drain the water in a cool and ventilated place, dry it at 50°C, and pass it through a 40-mesh sieve to obtain clematis powder.

[0047] (2) Accurately weigh a certain amount of clematis whole herb powder 50.00g, soak in 10 times the volume (v:w) of 95% ethanol for 12 hours, filter, repeat the treatment twice, combine the two filtrates to concentrate for later use; the filter residue is placed in a cool place Ethanol was evaporated in a ventilated place, and then dried in an oven at 50°C to obtain a clematis pretreatment powder.

[0048] (3) Accurately weigh 20.00 g of Clematis clematis pretreated powder, add distilled water at a liquid-to-solid ratio of 1:30 (g:mL), bathe in water at 70°C for 90 minutes, filter with suction, and extract repeatedly 4 times. Combine 4 filtrate polysaccharide extracts.

[0049] (4) The polysaccharide extract is fixed to volume, and the total sugar and reducing sugar content in the extract are det...

Embodiment 3

[0051] (1) Take the whole clematis herb, wash it, drain the water in a cool and ventilated place, dry it at 50°C, and pass it through a 40-mesh sieve to obtain clematis powder.

[0052] (2) Accurately weigh a certain amount of clematis whole herb powder 50.00g, soak in 10 times the volume (v:w) of 95% ethanol for 12 hours, filter, repeat the treatment twice, combine the two filtrates to concentrate for later use; the filter residue is placed in a cool place Ethanol was evaporated in a ventilated place, and then dried in an oven at 50°C to obtain a clematis pretreatment powder.

[0053] (3) Accurately weigh 25.00 g of Clematis clematis pretreated powder, add distilled water at a liquid-to-material ratio of 1:30 (g:mL), bathe in water at 70°C for 90 minutes, filter with suction, and extract repeatedly 4 times. Combine 4 filtrate polysaccharide extracts.

[0054] (4) The polysaccharide extract is fixed to volume, and the total sugar and reducing sugar content in the extract are ...

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Abstract

The invention discloses a method for extracting Anoectochilus roxburghii polysaccharides. The method comprises the steps: (1) cleanly washing Anoectochilus roxburghii herbs, then, baking the Anoectochilus roxburghii herbs at the temperature of 40-60 DEG C, then, crushing and sieving the baked Anoectochilus roxburghii herbs, soaking the sieved Anoectochilus roxburghii herbs with 80-95% ethanol according to the ratio (v: w) of (8-12): 1 for 10-15 hours, carrying out filtration, carrying out repeated treatment for 2-3 times, concentrating the filtrate for later use, and baking filtration residue so as to obtain pretreated powder; and (2) weighing the pretreated powder, adding distilled water into the weighed pretreated powder according to the liquid/material ratio of 1: (25-35), carrying out water bath for 60-90 minutes at the temperature of 60-70 DEG C, carrying out suction filtration, carrying out repeated extraction for 3-5 times, and merging the filtrates, thereby obtaining a polysaccharide extract. Under the condition of the extraction process disclosed by the invention, the extraction yield of the Anoectochilus roxburghii polysaccharides is 26.68%. By applying the process disclosed by the invention, the polysaccharides are high in yield and simple and easy in regulation and control and have production and application values.

Description

technical field [0001] The invention relates to a method for extracting clematis polysaccharides. Background technique [0002] Anoectochilusroxburghii (Wall.) Lindl., also known as golden clematis, golden grass, golden thread, golden earrings, money grass, etc. It is a perennial herbaceous plant of the genus Anoectochilus in the family Orchidaceae [1] . It is a traditional precious medicinal material in my country, and is known as the "King of Medicine" and "Golden Grass" among the people. Its taste is sweet and slightly bitter, and its nature is flat and slightly cold. It has the effects of clearing heat and cooling blood, expelling wind and dampness, strengthening the heart, strengthening the kidney, diuresis, calming the liver, and lowering blood pressure. It is mainly used to treat high blood pressure, diabetes, heart disease, and pneumonia. , acute and chronic hepatitis, nephritis and other diseases [2] . Polysaccharides are one of the important active ingredients...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 曾碧玉苏明华常强王伟李惠华
Owner SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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