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Method for making artificial seeds of star anise lotus

A technology of artificial seeds and production methods, applied in gardening methods, botanical equipment and methods, gardening tools/equipment, etc., to achieve the effects of overcoming easy browning, remarkable effect, and high frequency of plant regeneration

Active Publication Date: 2017-04-19
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no relevant report about the artificial seeds of star anise lotus both at home and abroad

Method used

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  • Method for making artificial seeds of star anise lotus
  • Method for making artificial seeds of star anise lotus
  • Method for making artificial seeds of star anise lotus

Examples

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example 1

[0047] 1. Making artificial seeds of star anise lotus

[0048] 1) Induction of somatic embryos of star anise lotus

[0049] Transfer the loose embryogenic callus of star anise lotus to the medium of differentiation medium MS+1.0mg / L 6-BA+2.0mg / LNAA. After 20 days of dark culture, the texture of the tissue structure is compact and the surface of the callus is formed Piles of looser nodule-like protrusions. With the extension of the dark culture time, most of the nodular protrusions differentiated into embryoid bodies, and a small amount differentiated into roots. The embryoid bodies are somatic embryos. The culture conditions are: dark culture, temperature 22 °C ± 2 ℃, culture period 20d.

[0050] 2) Liquid proliferation culture of somatic embryos

[0051] Transfer the 4th passage embryoid body to the liquid medium of MS+1.0mg / L 6-BA+1.0mg / L NAA+2.0mg / L 2,4-D+0.5mg / L ABA for suspension culture , while stirring with a mixer. Each 250mL Erlenmeyer bottle contains 100mL of cu...

Embodiment 2

[0066] 1. Making artificial seeds of star anise lotus

[0067] 1) Induction of somatic embryos of star anise lotus

[0068] Transfer the loose embryogenic callus of star anise lotus to the medium of differentiation medium MS+0.8mg / L 6-BA+1.5mg / LNAA. After 20 days of dark culture, the texture of the tissue structure is compact and the surface of the callus is formed Piles of looser nodule-like protrusions. With the extension of the dark culture time, most of the nodular protrusions differentiated into embryoid bodies, and a small amount differentiated into roots. The embryoid bodies are somatic embryos. The culture conditions are: dark culture, temperature 22 °C ± 2 ℃, culture period 15-20d.

[0069] 2) Liquid proliferation culture of somatic embryos:

[0070] The embryoid bodies of the third generation were transferred to the liquid medium of MS+0.8mg / L 6-BA+0.8mg / L NAA+1.5mg / L2, 4-D+0.4mg / L ABA for suspension culture, Meanwhile use a mixer to stir. Each 250mL Erlenmeyer ...

Embodiment 3

[0084] 1. Making artificial seeds of star anise lotus

[0085] 1) Induction of somatic embryos of star anise lotus

[0086] The loose embryogenic callus of Star Anise was transferred to the medium of differentiation medium MS+0.5mg / L 6-BA+1mg / LNAA. After 20 days of dark culture, the texture of the tissue structure was compact, and a layer of callus formed on the surface of the callus. Pile of looser nodular protrusions. With the extension of the dark culture time, most of the nodular protrusions differentiated into embryoid bodies, and a small amount differentiated into roots. The embryoid bodies are somatic embryos. The culture conditions are: dark culture, temperature 22 °C ± 2 ℃, culture period 15-20d.

[0087] 2) Liquid proliferation culture of somatic embryos:

[0088] Transfer the embryoid body of passage 4 to the liquid medium of MS+0.5mg / L 6-BA+0.5mg / L NAA+1mg / L 2,4-D+0.2mg / LABA for suspension culture, and at the same time Blend using a blender. Each 250mL Erlenme...

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Abstract

The invention discloses a production method of dysosma versipellis artificial seeds. The production method comprises the following steps: carrying out differentiation culture on dysosma versipellis embryonic callus to obtain a somatic embryo; carrying out multiplication culture on the somatic embryo to obtained multiplied somatic embryos; covering the obtained multiplied somatic embryos with an embedding agent to obtain the dysosma versipellis artificial seeds; and finally, spraying a 2.5-40g / L pullulan solution on the dysosma versipellis artificial seeds and storing to improve the germination rate of the artificial seeds. With the adoption of the production method, by using a modern biotechnology, situations that dysosma versipellis wild resources nearly run out and germchits are lacked in a large-scale artificial culture process are effectively alleviated; and the requirements on dysosma versipellis in daily life of people are met. After the embryonic callus is differentiated to obtain the somatic embryo and the seeds are produced, so that production steps are reduced and the production period is shortened. After the artificial seeds are sprayed by the pullulan solution, the germination rate of the seeds is improved.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically, the invention relates to a method for making artificial seeds of star anise lotus. Background technique [0002] Dysosma versipellis (Hance) M.Cheng is one of the traditional traditional Chinese medicines commonly used in my country. It has the effects of clearing heat and detoxifying, resolving phlegm and dissipating stagnation, eliminating phlegm and reducing swelling. Its main active ingredient is a natural product with anti-tumor activity-podophyllotoxin , a precursor of artificially synthesized drugs such as etoposide (VP-16) and teniposide (teniposide, VM-26). Clinical studies in recent years have shown that VP-16 and VM-26 have superior clinical effects and are important drugs for the treatment of testicular cancer, lymphoma, leukemia, and small cell lung cancer; and in vitro experiments have shown that VP-16 can enhance the anticancer effect of anticancer drugs. Effects of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 谭小明周雅琴余丽莹周小雷韦莹
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS