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Bacterial protein peptide that can replace fish meal and its preparation method and application in aquaculture

A technology to replace fishmeal and protein peptides, applied in the field of microorganisms and aquaculture feed, can solve the problems of protein source shortage and low protein utilization rate, and achieve the effects of easy promotion and use, strong practical operability, and multi-small peptide output

Active Publication Date: 2018-08-07
江苏省苏微微生物研究有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The main purpose of the present invention is to provide a new type of fungus protein that can more effectively promote animal growth and improve feed utilization in view of the current shortage of feed protein sources in my country, the current situation of low protein utilization, and the deficiencies of existing technologies and products. Peptide Products

Method used

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  • Bacterial protein peptide that can replace fish meal and its preparation method and application in aquaculture
  • Bacterial protein peptide that can replace fish meal and its preparation method and application in aquaculture
  • Bacterial protein peptide that can replace fish meal and its preparation method and application in aquaculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Preparation of Mycofurfurin Peptide

[0054] (1) Bacteria chaff pretreatment

[0055] Crush 2000g of edible fungus chaff and remove visible impurities, then transfer to an oven, dry at 50°C until the water content is less than 5%, crush, pass through a 40-mesh sieve, and set aside.

[0056] (2) Bacteria chaff fermentation

[0057] ① Preparation of culture medium

[0058] PDA slant medium: Weigh 200g of potatoes, peel them, cut them into small pieces, add 1L of deionized water and boil for 30 minutes, add deionized water appropriately during the boiling process; then filter, and dilute the obtained filtrate to 1L with deionized water ; Then add 20g of glucose, 20g of agar, and the pH is natural. Sterilize at 121°C for 30 min to make a slope.

[0059] Malt juice agar medium: Weigh 145.1 g of malt juice agar medium, add 1 L of deionized water, stir, heat and boil until completely dissolved, sterilize at 115°C for 15 minutes, and make a slope.

[0060] Ganode...

Embodiment 2

[0072] The mensuration of embodiment 2 mycofurfurin peptide

[0073] (1) Determination of molecular weight distribution

[0074]Weigh 20mg of mycofurfurin peptide powder into a 10mL volumetric flask, use the mobile phase (acetonitrile: water: trifluoroacetic acid = 45: 55: 0.1, volume ratio) to adjust the volume to the mark, and ultrasonically oscillate for 10 minutes to fully dissolve the sample. 0.22μm nylon filter membrane, and analyzed by gel chromatography. Analysis conditions: Waters1525, chromatographic column TSK gel G2000 SWXL 300mm×7.8mm; mobile phase is acetonitrile: water: trifluoroacetic acid = 45: 55: 0.1 (volume ratio); detection wavelength UV 220nm; flow rate 0.5mL / min, column temperature 30°C, the injection volume is 10 μL, the column calibration of the gel chromatographic column is not less than 5000 according to the peak calculation of the standard (tyrosine-thyrosine-tyrosine-arginine), the distribution coefficient of the oligopeptide (Kd ) between 0 and ...

Embodiment 3

[0084] Example 3: Feeding crucian carp with mycofurin peptide

[0085] The experiment was completed in the laboratory of the Freshwater Fishery Research Center of the Chinese Academy of Fishery Sciences. Fish species are provided by the central fishery. A total of 540 crucian carp with the same health, size and weight were selected, and the initial weight of the individual was (20±0.5) g. The fish species were randomly divided into 6 groups, with 3 replicates in each group, and 30 fish in each replicate, and were stocked in cages with a size of 1.00 m×1.00 m×1.00 m in replicate units.

[0086] The basic feed was formulated with reference to the Chinese aquatic industry standard SC / T 074-2004. The control group was fed with basal feed (10% fish meal, no mycofurin peptide); the test group was fed with mycofurin peptide in the basal feed, and the corresponding amount of fish meal was subtracted at the same time, of which: test group 1 (fish meal 8.4%, bacteria furfur protein p...

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Abstract

The invention provides a mushroom bran protein peptide capable of replacing fish meal and a preparation method and application of the mushroom bran protein peptide in aquaculture, and belongs to the technical field of microorganisms and aquaculture feed. Edible mushroom bran waste serves as a basic raw material, firstly, the step-by-step solid fermentation technology in which ganoderma lucidum is used firstly and then brewer yeast is used is adopted, original nutrients of edible mushroom bran are fully converted into high-quality bacterial protein and polysaccharides; then, the enzymolysis technology combining cellulose, edible mushroom hydrolase and yeast extracted enzyme is adopted, the protein is fully released and hydrolyzed into low-molecular-weight small peptides; finally, the mushroom bran protein peptide product rich in the small peptides easy to digest and high in DPPH free radical removal capacity is obtained through concentration and drying. The preparation method of the mushroom bran protein peptide has the advantages of being high in utilization rate of mushroom bran, high in product yield, easy to operate, high in practicality and convenient to popularize. The mushroom bran protein peptide is used for aquaculture feed, can partly replace the fish meal, and has the functions of promoting growth of aquatic animals and increasing the feed utilization rate.

Description

technical field [0001] A mycofur protein peptide that can replace fish meal and its preparation method and application in aquaculture. The present invention relates to a mycofur protein hydrolyzate product with relatively high short peptide content and antioxidant capacity, its production and preparation method and The application of the fishmeal substitute in aquaculture belongs to the technical field of microorganisms and aquaculture feed. Background technique [0002] Mushroom bran is the use of straw, wood chips and other raw materials for the cultivation of edible fungus bags, and the leftovers of the culture medium after receiving the goods are commonly known as edible fungus cultivation waste or fungus residue. In recent years, with the expansion of the production scale of edible fungi and the improvement of output in my country, edible fungi have become the sixth largest agricultural product after grain, cotton, oil, fruit and tea. According to statistics from the C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/80A23K20/147
CPCY02A40/818
Inventor 张东升张一平何静霞周群兰汪洁王红连蒋益全卫丰匡群
Owner 江苏省苏微微生物研究有限公司
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