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Umbelopsis nana and application thereof in promoting growth of Dendrobium officinale

A technology for dwarf agaric mold and Dendrobium officinale, which is applied in the field of microorganisms, can solve the problems of slow growth rate and low average yield of Dendrobium officinale, and achieves the effects of good development and application prospects, easy preservation, and easy industrial production.

Inactive Publication Date: 2015-12-02
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As wild Dendrobium officinale resources are on the verge of depletion, at present, the market demand of Dendrobium candidum mainly depends on artificial cultivation, but the growth rate of artificially planted Dendrobium candidum is slow and the average yield is low

Method used

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  • Umbelopsis nana and application thereof in promoting growth of Dendrobium officinale
  • Umbelopsis nana and application thereof in promoting growth of Dendrobium officinale
  • Umbelopsis nana and application thereof in promoting growth of Dendrobium officinale

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Embodiment 1

[0020] The mycorrhizal fungus Umbelopsisnana JSNL001-4 was prepared into a solid microbial agent. The specific preparation method is as follows: use cottonseed shell fungus residue as the culture substrate, ammonium nitrate as the exogenous nitrogen source, and sucrose as the exogenous carbon source, prepare a solid culture substrate and put it in an autoclave bag, and heat it at 121°C and 101.3 kPa Autoclave for 20 minutes. After cooling the culture medium, inoculate the mycorrhizal fungus JSNL001-4 under aseptic conditions, and then carry out artificial culture. The specific culture conditions are 26° C., 12 hours of light + 12 hours of darkness, pH 6.5, and cultured for 25 to 30 days. Process the cultured bacterial agent into 1cm 3 Blocks of the same size are placed on the surface of the Dendrobium officinale seedling cultivation substrate planted in that year. Cultivate Dendrobium candidum is selected the nutrient bowl of diameter 15cm, and each nutrient bowl is cultiva...

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Abstract

The invention discloses Umbelopsis nana, classified under Umbelopsis in Mucorales in Zygomycotina, under a bacterial number JSNL001-4, already collected in china general microbiological culture collection center under CGMCC No.11308 on 11th Sep., 2015. The Umbelopsis nana JSNL001-4 is capable of promoting growth of Dendrobium officinale, has evident effect, is suitable for artificial cultivation, is quick to breed and convenient to produce on a large scale and has a promising development and application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an agaricus dwarf and its application in promoting the growth of Dendrobium officinale. Background technique [0002] Dendrobium officinale is a perennial epiphytic herb of the genus Dendrobium orchidaceae. Studies have shown that from the germination of its seeds to the healthy growth of the plant, the participation of symbiotic fungi is all necessary. As wild Dendrobium officinale resources are on the verge of depletion, at present, the market demand of Dendrobium officinale mainly depends on artificial cultivation, but artificial cultivation of Dendrobium officinale has a slow growth rate and low average yield. Mycorrhizae are symbionts with specific morphological structures and functions formed by mycorrhizal fungi and plant roots in soil, and are a common symbiotic phenomenon in nature. Orchids are highly dependent on mycorrhizae. Mycorrhizal fungi and pl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
Inventor 徐超贾君汪善峰张红岩郑凯杨鹤同席刚俊史俊赵桂华
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY