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A kind of Fusarium oxysporum and its application in promoting the growth of Dendrobium officinale

A technology of Fusarium oxysporum and Dendrobium candidum, applied in the field of microorganisms, can solve the problems of slow growth and low average yield of Dendrobium candidum, and achieve good development and application prospects, easy preservation, and easy industrial production.

Active Publication Date: 2018-03-30
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

As wild Dendrobium officinale resources are on the verge of depletion, at present, the market demand of Dendrobium candidum mainly depends on artificial cultivation, but the growth rate of artificially planted Dendrobium candidum is slow and the average yield is low

Method used

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  • A kind of Fusarium oxysporum and its application in promoting the growth of Dendrobium officinale
  • A kind of Fusarium oxysporum and its application in promoting the growth of Dendrobium officinale
  • A kind of Fusarium oxysporum and its application in promoting the growth of Dendrobium officinale

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Embodiment 1

[0020] The mycorrhizal fungus Fusarium oxysporum (Fusarium oxysporum) JSNL004 was prepared into a solid microbial agent. The specific preparation method is as follows: use cottonseed shell fungus residue as the culture substrate, ammonium nitrate as the exogenous nitrogen source, and sucrose as the exogenous carbon source, prepare a solid culture substrate and put it in an autoclave bag, and heat it at 121°C and 101.3 kPa Autoclave for 20 minutes. After cooling the culture medium, inoculate the mycorrhizal fungus JSNL004 under aseptic conditions, and then carry out artificial cultivation. The specific culture conditions are 26° C., 12 hours of light + 12 hours of darkness, pH 6.5, and cultured for 25 to 30 days. Process the cultured bacterial agent into 1cm 3 Blocks of the same size are placed on the surface of the Dendrobium officinale seedling cultivation substrate planted in that year. Cultivate Dendrobium candidum is selected the nutrient bowl of diameter 15cm, and each...

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Abstract

The invention discloses fusarium oxysporum, which has a classification name of fusarium oxysporum. The strain number is JSNL004; the fusarium oxysporum is preserved in CGMCC (China General Microbiological Culture Collection Center) of China microbe preservation management committee; the preservation number is CGMCC No.11310; and the preservation date is September 10, 2015. The JSNL004 has the obvious effect in aspects of accelerating the growth speed of dendrobium officinale and the like; the artificial culture can be realized; the reproduction speed is high; the mass production can be conveniently realized; and good development and application prospects are realized.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Fusarium oxysporum and its application in disease resistance of dendrobium officinale. Background technique [0002] Dendrobium officinale is a perennial epiphytic herb of the genus Dendrobium orchidaceae. Studies have shown that from seed germination to healthy growth of seedlings, there is no need for the participation of symbiotic fungi. As wild Dendrobium officinale resources are on the verge of depletion, at present, the market demand of Dendrobium officinale mainly depends on artificial cultivation, but artificial cultivation of Dendrobium officinale has a slow growth rate and low average yield. Mycorrhizae are symbionts with specific morphological structures and functions formed by mycorrhizal fungi and plant roots in soil, and are a common symbiotic phenomenon in nature. Orchids are highly dependent on mycorrhizae. Mycorrhizal fungi and plant roots f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/77
Inventor 徐超刘国华蒋为民贾君汪善峰张红岩郑凯席刚俊史俊
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY