Fusarium oxysporum and application of fusarium oxysporum to growth promotion of dendrobium officinale
A technology for Fusarium oxysporum and Dendrobium officinale, which is applied in the field of microorganisms, can solve the problems of low average yield and slow growth rate of Dendrobium officinale, and achieves the effects of easy preservation, good development and application prospects, and simple culture conditions.
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[0020] The mycorrhizal fungus Fusarium oxysporum (Fusarium oxysporum) JSNL004 was prepared into a solid microbial agent. The specific preparation method is as follows: use cottonseed shell fungus residue as the culture substrate, ammonium nitrate as the exogenous nitrogen source, and sucrose as the exogenous carbon source, prepare a solid culture substrate and put it in an autoclave bag, and heat it at 121°C and 101.3 kPa Autoclave for 20 minutes. After cooling the culture medium, inoculate the mycorrhizal fungus JSNL004 under aseptic conditions, and then carry out artificial cultivation. The specific culture conditions are 26° C., 12 hours of light + 12 hours of darkness, pH 6.5, and cultured for 25 to 30 days. Process the cultured bacterial agent into 1cm 3 Blocks of the same size are placed on the surface of the Dendrobium officinale seedling cultivation substrate planted in that year. Cultivate Dendrobium candidum is selected the nutrient bowl of diameter 15cm, and each...
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