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Corynebacterium guangdongensis producing various amino acids and application of Corynebacterium guangdongensis

A technology of coryneform bacteria and threonine, applied in the field of microorganisms, can solve the problems of poor production efficiency, low acid production rate of strains, and less amino acids

Active Publication Date: 2015-12-02
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The overall level of my country's amino acid production technology is far from the international advanced level, especially in the low acid production rate of strains, poor production efficiency, and few high-quality and high-value amino acids.

Method used

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  • Corynebacterium guangdongensis producing various amino acids and application of Corynebacterium guangdongensis
  • Corynebacterium guangdongensis producing various amino acids and application of Corynebacterium guangdongensis
  • Corynebacterium guangdongensis producing various amino acids and application of Corynebacterium guangdongensis

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Embodiment 1

[0012] 1. Isolation of Corynebacterium Guangdong S01.

[0013] Corynebacterium guangdongensis S01 of the present invention is isolated from the NA flat plate of the laboratory pollution of the ultra-clean bench of the laboratory of the Guangdong Institute of Microbiology, and the NA flat plate has polluted light yellow colonies during placement, which is picked and cultivated and identified The Corynebacterium cantonensis S01 described in the present invention was obtained during the light yellow colony process.

[0014] The formula of the NA medium is: 10.0 g of peptone, 3.0 g of beef extract, 5.0 g of sodium chloride, 15.0 g of agar, 1000 mL of double distilled water, pH 7.3, after mixing evenly, sterilize for later use.

[0015] 2. Identification of Corynebacterium Guangdong S01.

[0016] 1. Phenotypic characteristics: Corynebacterium guangdong S01 was inoculated on NA medium with a three-section line, placed in a 30°C incubator for 2 days, and taken out to observe the cha...

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Abstract

The invention discloses Corynebacterium guangdongensis and application of the Corynebacterium guangdongensis. Corynebacterium guangdongensis is collected at China General Microbiological Culture Collection Center (CGMCC), in the Institute of Microbiology of the Chinese Academy of Sciences in Chaoyang District of Beijing, China and under number of CGMCC No:11162. Corynebacterium guangdongensis S01 can produce 15 amino acids including aspartic acid, threonine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine and arginine, thereby having important application value in developing amino acid products.

Description

Technical field: [0001] The invention belongs to the technical field of microbes, and in particular relates to a new bacterial species capable of producing multiple amino acids—Crynebacterium guangdongensis and its application. Background technique: [0002] Amino acid is the basic unit of biological protein and has irreplaceable biological functions. It has been widely used in medicine, health care, food, feed, fertilizer, cosmetics and other fields. Sodium glutamate is the first amino acid used by humans, and it is also the most widely used amino acid with the largest production and sales in the world. In recent years, the international pharmaceutical, food, feed and other industries have developed rapidly, and the demand for amino acids is increasing. At present, there are nearly 100 amino acid production enterprises in my country, with a total output of more than 3 million tons. Among them, the annual output of glutamic acid and its salts, a bulk amino acid product, has...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/20C12P13/08C12P13/14C12P13/04C12P13/06C12P13/12C12P13/22C12P13/24C12P13/10C12R1/15
Inventor 羊宋贞朱红惠冯广达李燕旋杨瑞恒
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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