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A kind of cadmium-low-density lipoprotein chelate and its preparation method and application
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A low-density lipoprotein and chelate technology, which is applied in the medical field to achieve the effect of improving accuracy and repeatability
Active Publication Date: 2018-08-21
湘潭市第一人民医院
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[0006] For the serious problem of cadmium pollution, the object of the present invention is to provide a kind of cadmium-low-density lipoprotein chelate and preparation method thereof, and establish the qualitative and quantitative detection method of cadmium-low-density lipoprotein chelate, so that quantitative detection Application of cadmium-low-density lipoprotein chelate in evaluating the degree of cadmium pollution in an area
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[0059] The present invention also provides the preparation method of cadmium-low density lipoprotein chelate as above, it comprises the following steps:
[0060] 1) Synthesis steps of low-density lipoprotein:
[0061] Adding cadmium ions to human low-density lipoprotein for chelation reaction;
[0062] 2) Purification steps of low-density lipoprotein:
[0063] Immunoaffinity chromatography is used to remove unreacted low-density lipoprotein and cadmium ions in the cadmium-low-density lipoprotein chelate obtained in step 1), thereby obtaining the cadmium-low-density lipoprotein chelate.
[0064] Described step 2) comprises the following steps:
[0065] A. redissolving the LDL-Cd chelate obtained in step 1) in normal saline to obtain a complex solution;
[0066] B. Equilibrium chromatography column: Use buffer to wash the chromatography column, pack the filler that can specifically bind to LDL, and then equilibrate the chromatography column with buffer;
[0067] C. Loading: ...
Embodiment 1
[0154] Example 1 : the preparation method one of cadmium-low-density lipoprotein chelate, it comprises the following steps:
[0155] 1) Synthesis steps of LDL-Cd chelate: adding cadmium ions to the purified low-density lipoprotein derived from human body for chelation reaction;
[0156] 2) Purification step of LDL-Cd chelate: use immunoaffinity chromatography to remove LDL and cadmium ions not involved in the reaction in the LDL-Cd chelate obtained in step 1), specifically as follows:
[0157] A. redissolving the LDL-Cd chelate obtained in step 1) in normal saline to obtain a complex solution;
[0159] C. Loading: Before loading, dilute the reconstituted solution with dilution buffer, and then load the sample, the LDL-Cd chelate and LDL that ha...
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Abstract
The invention discloses a cadmium-low-density lipoprotein chelate and its preparation method and application. The cadmium-low-density lipoprotein chelate is composed of cadmium ions through zincfinger structure, thiol and cysteine residues. At least one structure of chelated with low-density lipoprotein. The invention establishes a qualitative and quantitative detection method for cadmium-low-density lipoprotein chelate, so as to quantitatively detect the application of cadmium-low-density lipoprotein chelate in evaluating the degree of cadmium pollution in an area. Quantitative detection of cadmium-low-density lipoprotein chelate in the serum of a population in an area can indirectly reflect the cadmium pollution of the population in this area, thereby indirectly reflecting the degree of cadmium pollution in this area. The cadmium-low-density lipoprotein chelate quantitative detection method established by the invention has greatly improved accuracy and greatly improved detection repeatability.
Description
technical field [0001] The invention relates to the medical field, in particular to a cadmium-low-density lipoprotein chelate and a preparation method and application thereof. Background technique [0002] Lipoprotein (lipoproteins) is a lipid-protein complex formed by the combination of protein and lipid, which can be divided into high density lipoprotein (high density lipoprotein, HDL), intermediate density lipoprotein (intermediate density lipoprotein, IDL) according to density , low density lipoprotein (low density lipoprotein, LDL), very low density lipoprotein (very low density lipoprotein, VLDL), chylomicron (chylomicron, CM). About 70% of the cholesterol in the blood is carried by LDL and VLDL, and the cholesterol content of LDL is greater than that of VLDL. Because LDL is rich in cholesterol, it is called "bad cholesterol", which is closely related to the occurrence and development of various diseases Related, especially coronary heart disease, diabetes and other d...
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