A kind of inoculation method of corn gray spot disease

Abstract

An inoculation method for corn gray spot disease, comprising the following steps: (1) transplanting corn plants at the trumpet-mouth stage; (2) cultivating in a real simulated environment in an artificial climate incubator; (3) collecting pathogenic bacteria; 4) inoculating. The invention is not limited by factors such as season, external environment and region, effectively improves the success rate of artificial inoculation of gray spot disease, can accurately control the inoculation time of gray spot disease, and is beneficial to observe and detect specific time nodes before and after inoculation of plants The appearance and internal indicators change dynamically.

Description

technical field [0001] The invention relates to an inoculation method for plant gray spot disease, in particular to an inoculation method for corn gray spot disease. Background technique [0002] Maize gray spot disease is one of the main diseases in corn production areas. As the damage caused by gray spot disease in corn production increases year by year, the research on corn gray spot disease is also deepening. In recent years, the research on corn gray spot resistance mainly covers the research on the pathogenic mechanism of gray spot disease, the pathogenicity of pathogenic bacteria, the identification of plant disease resistance, and the location and cloning of resistance genes. It is used to realize the infection of corn plants by pathogenic bacteria through natural inoculation or artificial inoculation, and then cause diseases, so that researchers can track and investigate the whole process of disease. [0003] At present, the occurrence of corn gray spot has high re...

AI Technical Summary

Problems solved by technology

This inoculation method cannot control the air temperature and relative humidity, thus affecting the inoculation effect; at the same time, the natural inoculation experimenters cannot accurately grasp the inoculation time and the infection occurrence time, and cannot observe and collect the physiological changes of infected plants at specific time points before inoculation and after inoculation. Changes in biochemical indicators

Manual inoculation requires the manual isolation and cultivation of the gray spot pathogenic bacteria. Under the environmental conditions of the field or greenhouse, the inoculation is completed by artificial spraying, injection of the carrier solution, or insertion of the carrier carrier into the plant body, and induces the disease of the plant. The inoculation Although the method can accurately control the inoculation time, and can also obtain the purified gray spot pathogenic bacteria through the steps of pathogenic bacteria isolation and cultivation, but the artificial inoculation of infected plants is limited by seasons and regions, and can only be used in specific seasons that meet the growth of corn (such as spring and summer). ) for field identification, the air temperature and relative humidity changes after plant inoculation cannot be controlled, the inoculation host is difficult to get sick, and the success rate of inoculation is not high; in addition, the gray spot pathogen grows slowly under artificial conditions, and it is easy to be polluted during the cultivation period. One of the reasons for limiting the success rate of artificial inoculation