Capsule having function of antioxidation and preparation method of capsule
An anti-oxidation and capsule technology, applied in the field of capsules with anti-oxidation function and its preparation, can solve the problem of not using a variety of marine active functional factors chitosan oligosaccharide and the like
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Embodiment 1
[0026] A kind of preparation method of chitosan oligosaccharide, concrete steps are as follows:
[0027] (1) Add chitosan / water to the high-speed shearing disperser at a ratio of 0.1Kg / L, and after running at 2000rpm for 3 minutes, add glacial acetic acid at a weight ratio of chitosan aqueous solution: glacial acetic acid=10:1; After running for 15 minutes, add 12000U / kg (chitosan) chitosan hydrolase, and control the temperature at 55°C;
[0028] (2) When the viscosity drops below 800cp and the pH is 4.8, control the stirring speed to 4000rpm, continuously add chitosan powder, and add it according to the volume ratio of 3% (100 parts of chitosan solution plus 3 parts of glacial acetic acid) Dissolve in glacial acetic acid, and add chitosan hydrolase according to the amount of chitosan hydrolase mentioned above, until the concentration of chitosan solution reaches 12%, pH5.0; then add chitosan hydrolase 12000U in the solution / kg (chitosan), at a temperature of 55°C, stirred a...
Embodiment 2
[0034] A capsule content with antioxidant function, and its antioxidant effect test evaluation is as follows:
[0035] Experimental animals: 90 12-month-old male Kunming mice of SPF grade (body weight 45.5-55.5 grams), each old mouse was weighed and tail blood was taken to measure MDA content, and randomly divided into 18 groups according to body weight and MDA content , 5 in each group, so that the levels of MDA in each group were basically the same.
[0036] Dosage: Feed according to the total recommended dosage of 2000mg / d of capsule contents for normal human body, continuously feed for 30 days, prepare 4% hemolysis and serum by collecting blood from tail tip before and after feeding, measure MDA level and SOD activity according to the kit instructions, and comfort The agent is glucose powder. The test group settings and analysis results are as follows:
[0037] Table 1 Compatibility Table 1 of Capsule Contents
[0038]
[0039] Table 2 The results of the antioxidant ...
Embodiment 3
[0051] A capsule content with antioxidant function, and its antioxidant effect test evaluation is as follows:
[0052] Experimental animals: 40 12-month-old male Kunming mice of SPF grade (body weight 45.5-55.5 grams), each old mouse was weighed and tail blood was taken to measure MDA content, and randomly divided into 4 groups according to body weight and MDA content , 10 in each group, so that the levels of MDA in each group are basically the same.
[0053] Dosage: Feed according to the total recommended dosage of 2000mg / d of capsule contents for normal human body, continuously feed for 30 days, prepare 4% hemolysis and serum by collecting blood from tail tip before and after feeding, and measure MDA level and SOD activity according to the kit instructions. According to embodiment 2 test result, test group setting and analysis result are as follows:
[0054] Table 5 Compatibility Optimization of Capsule Contents III
[0055]
[0056] Table 6 The results of the antioxidant...
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