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Bacillus altitudinis YLX-5 and application thereof

A technology of Bacillus alpina and YLX-5, applied in the field of selenophilic microorganisms and its applications, can solve the problems of unidentified microorganism species and characteristics

Active Publication Date: 2015-12-09
安徽硒谷生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the invention patent did not isolate and purify related selenium-tolerant microorganisms, nor did it identify the species and characteristics of the microorganisms

Method used

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  • Bacillus altitudinis YLX-5 and application thereof
  • Bacillus altitudinis YLX-5 and application thereof
  • Bacillus altitudinis YLX-5 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The application of the microorganism described in the invention in the synthesis of organic selenium.

[0045] Inoculate the microorganism YLX-5 strain of the present invention into the TSB culture solution containing selenium (sodium selenite configuration) 10mg / L, cultivate it at 200r / min for 48 hours at normal temperature, and collect the bacteria and supernatant by centrifugation. Then, liquid chromatography-atomic fluorescence spectrometry (LC-GA-AFS) was used to detect the content and form of selenium in the supernatant and bacteria, and the results showed that almost all of the supernatant was Se 4+ , and most of the cells are selenomethylselenocysteine ​​(SeMeCys) (Table 4). This shows that YLX-5 bacterial strain can under 10mgSe / L condition, the inorganic selenium (Se 4+ ) into organic selenium (SeMeCys) and accumulate in the bacteria. Further collection of bacteria can be used as organic selenium for animal feed, etc., and can also be used as raw material fo...

Embodiment 2

[0050] The application of the microorganism described in the invention in the synthesis of organic selenium.

[0051] Inoculate the microorganism YLX-5 strain of the present invention into the TSB culture solution containing selenium (sodium selenite configuration) 20 mg / L, cultivate it at 200 r / min for 72 hours at normal temperature, and collect the bacteria and supernatant by centrifugation. Then, liquid chromatography-atomic fluorescence spectrometry (LC-GA-AFS) was used to detect the content and form of selenium in the supernatant and bacteria, and the results showed that almost all of the supernatant was Se 4+ , and most of the bacteria are methyl selenocysteine ​​(SeMeCys), and a small amount of Se 4+ (table 5). This shows that YLX-5 bacterial strain can under the condition of 20mgSe / L, the inorganic selenium (Se 4+ ) into organic selenium (SeMeCys) and accumulate in the bacteria. Further collection of bacteria can be used as organic selenium for animal feed, etc., an...

Embodiment 3

[0056] The application of the microorganism described in the invention in the synthesis of nanometer selenium.

[0057] Microbe YLX-5 strain of the present invention is inoculated in the TSB culture fluid that contains selenium (sodium selenite configuration) 200mg / L, cultivates 24 hours at 200r / min under normal temperature, and the solution appears obvious red, turbid. After culturing for 48 hours, the cells and supernatant were collected by centrifugation, and it was found that the collected precipitate was a red suspension. Then, using liquid chromatography-atomic fluorescence spectrometry (LC-GA-AFS) and atomic fluorescence spectrometer, it was identified that the composition of the red suspension was almost elemental selenium by subtraction method. The nano-selenium synthesized by the microorganism can be used as a source of selenium supplementation for selenium-deficient people, and can also be used as a selenium fertilizer or an animal feed additive.

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Abstract

The invention discloses bacillus altitudinis YLX-5 separated from a microbial mat sediment in a percolating water system in the selenium mining area of Enshi, Hubei, China and application of bacillus altitudinis YLX-5. The bacillus altitudinis YLX-5 is preserved in CCTCC (China Center for Type Culture Collection) with the preservation number of CCTCC No: M2013675. The bacillus altitudinis YLX-5 has the characteristics of selenium-addicted microbes and has a potential application value in microbial organic selenium conversion, microbial nano-selenium synthesis, selenium-containing ore microbial activation, selenium-rich microbial fertilizer preparation and selenium-contaminated environment remediation.

Description

technical field [0001] The present invention relates to a selenophilic microorganism and its application, in particular to a strain of Bacillus altitudinis YLX-5 and its use in microbial transformation and synthesis of organic selenium, microbial synthesis of nano-selenium, microbial activation of selenium ore, selenium-rich microbial fertilizer and selenium-polluted environment restoration The application in belongs to the field of biotechnology. Background technique [0002] Extremophiles refer to microbial species suitable for living in extreme environments, including thermophilic bacteria, halophilic bacteria, alkaliphilic bacteria, acidophilic bacteria, barophilic bacteria, psychrophilic bacteria, and radiation-resistant, dry-resistant, and high-concentration metal-resistant bacteria. Ion and extreme anaerobic microorganisms (Rothschild&Mancinelli, 2001; Chen Jun et al., 2006). At present, the research on extremophiles is mainly in areas such as mid-ocean ridges, hot sp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12P13/12C12P3/00C05F11/08C02F3/34C12R1/07
Inventor 袁林喜
Owner 安徽硒谷生物科技有限公司
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