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A kind of substituted urea herbicide residue degrading bacterial strain and its production

A herbicide and urea-substituting technology is applied in the field of substituted urea herbicide residual degrading strains and the inoculum produced therefrom, which can solve problems such as being difficult to remove, and achieves protection of health, low production and use costs, and good removal effect. Effect

Active Publication Date: 2018-03-09
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since pesticide residue pollution is generally a non-point source pollution, which is widespread and stubborn, it is difficult to completely remove it by general methods

Method used

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  • A kind of substituted urea herbicide residue degrading bacterial strain and its production
  • A kind of substituted urea herbicide residue degrading bacterial strain and its production
  • A kind of substituted urea herbicide residue degrading bacterial strain and its production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, isolation and identification of bacterial strain

[0042] The invention provides a bacterial strain capable of efficiently degrading urea herbicides and the bacterial agent produced therefrom. The bacterial strain used is Gram-negative bacteria LR2014-1, which is isolated from the soil of a pesticide factory in Rudong, Jiangsu. The specific isolation and screening methods for strains are as follows:

[0043] Take 5.0g of soil sample and add it to 100ml of liquid inorganic salt medium (hereinafter referred to as MM) containing 0.2mM Liguron, culture it on a shaker at 30°C and 150rpm for 7 days, and transfer it to fresh In the same medium, four consecutive enrichment cultures were carried out. Dilute the fifth-generation enrichment solution on MM solid medium containing 0.2mM Ligulong, culture at 30°C for 5 days, pick a single colony on the plate and put it in 3ml liquid LB test tube medium, then save and transfer Into 20mL MM medium containing 0.2mM Rigur...

Embodiment 2

[0045] Embodiment 2, laboratory degradation experiment

[0046] 2.1 Preparation of seed solution

[0047] Insert the strain LR2014-1 into 100mL LB medium containing 0.2mM Liguron, cultivate it on a shaker at 30°C and 150rpm, collect the bacteria by centrifugation at 6000rpm after 48h, wash the bacteria twice with MM, and finally resuspend with 10mL MM , as a seed solution for later use.

[0048] 2.2 Degradation of the herbicide Liguron by strain LR2014-1

[0049] The strain LR2014-1 was inoculated into 100mL MM containing 0.2mM Liguron at 5% inoculation amount, cultured on a shaker at 30°C and 150rpm, and 5mL was sampled every 3 hours for up to 24 hours. The residual amount of Riguron was detected, the degradation rate was calculated, and the time-degradation curve of strain LR2014-1 to Riguron herbicide was drawn. Such as figure 2 , strain LR2014-1 can completely degrade 0.2mM Riguron within 24h.

[0050] 2.3 Effect of temperature on degradation of Ligulong by strain LR...

Embodiment 3

[0066] Embodiment 3, soil degradation experiment

[0067] The vegetable garden soil was taken as the test soil sample. Pass the soil sample through a 2mm sieve, take a certain amount of Liguron, Diuron and Propannil powders and dissolve them in 10mL of methanol, and then soak in diatomaceous earth to completely absorb the pesticides. The soaked diatomaceous earth is placed in a fume hood to dry, and it is mixed into the soil so that the concentration of the pesticide in the soil is about 2mg / kg. 500g of each soil sample was cultured in a constant temperature incubator at 30°C, and the inoculum was inserted into the seed solution at an inoculation amount of 10%, and the soil without inoculation was used as a control, and the water holding capacity of the soil was maintained at 60%. After culturing for 7 days, the residual amount was determined by HPLC. The measurement results are shown in Table 3.

[0068] It can be concluded from Table 3 that after 7 days of cultivation, th...

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Abstract

The invention provides a phenyl urea-group herbicide residue degradation strain and a microbial inoculum produced by the strain, wherein the strain LR2014-1 is preserved in China Center for type Culture Collection on April 15, 2015 with preservation number of CCTCC M2015236. The phenyl urea-group herbicide residue degradation strain LR2014-1 disclosed by the invention can be applied to the degradation of phenyl urea-group herbicides, and preferably to the degradation of phenyl urea-group herbicides in soil. The degradation microbial inoculum product can be directly applied so that linuron residue in the soil can be reduced by more than 85%, therefore the problem of excessive residue of the phenyl urea-group herbicides in agricultural production is solved, phytotoxicity of herbicide residue on after-reap crops is prevented and non-toxic and pollution-free green agricultural products are produced.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a urea herbicide residue-degrading bacterial strain and a bacterial agent produced therefrom. technical background [0002] The extensive use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by diseases, insect pests and weeds for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production. played an immeasurable role. But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause serious harm to the ecological system. Extensive use of pesticides has caused pesticide residues in agricultural products to exceed the standard, seriously affecting people's health. With the improvement of people's living standards an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 蒋建东张龙杜方岭周庆新王军华陈凯李顺鹏
Owner NANJING AGRICULTURAL UNIVERSITY
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