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Enhanced acridinium ester luminescence system

A technology of enhancing luminescence and acridinium ester, which is applied in the direction of chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, can solve problems such as easy generation of bubbles, large variation of measurement results, and reduction of luminous intensity enhancement effects. Achieve the effects of lowering the freezing point, enhancing the luminous intensity, and reducing the chance of air bubbles

Active Publication Date: 2015-12-16
XIAMEN INNODX BIOTECH CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This increases the water solubility of the electronically excited state intermediates in the entire reaction system, but at the same time reduces the surface tension of the luminescent system, making it easier for the entire system to generate bubbles, which will affect the photometry, thereby reducing the impact on the luminous intensity. At the same time, the generation of bubbles also introduces a large variation in the photometry system, resulting in a large variation in the measurement results

Method used

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  • Enhanced acridinium ester luminescence system
  • Enhanced acridinium ester luminescence system
  • Enhanced acridinium ester luminescence system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] with 1% H 2 o 2 The solution is the pre-excitation solution, and the solution containing 1mol / L NaOH is used as the excitation solution as the basic light-emitting system. 5mmol / LEDTA is added to the system, and 0.125% or 0.25% or 0.5% or 1% of TritonX- 100 (TX-100), Tween-20, Dimethylformamide (DMF), and Ethanol. Add 10 μL diluted acridinium ester (AE) and anti-alpha-fetoprotein (AFP) antibody conjugate (AE content is about 5×10 -8 mmol / L), the relative luminous intensity of each formula was measured with a single-tube chemiluminescent instrument siriusL (set detection time to 0.8s), and the relative luminous intensity of the previous basic luminous system was 100%, and each group of formulas was calculated respectively The relative luminous intensity and relative activity are shown in Table 1 below:

[0018] Table 1 Effect of different additives on the relative luminous intensity of acridinium esters

[0019]

[0020]

[0021] The results in the table show t...

Embodiment 2

[0023] with 1% H 2 o 2 The solution is the pre-excitation solution, and the solution containing 1mol / L NaOH is used as the excitation solution, as the basic light-emitting system, and 5mmol / LEDTA is added to the system. On the basis of Example 1, TX-100 and Tween-20 as enhancers are paired and combined with DMF and ethanol as stabilizers to examine the impact of the addition of stabilizers on the effect of enhancers, and to screen the light-emitting system. Final recipe. Add 10 μL diluted acridinium ester (AE) and anti-alpha-fetoprotein (AFP) antibody conjugate (AE content is about 5×10 -8 mmol / L), the relative luminous intensity of each formula was measured with a single-tube chemiluminescent instrument siriusL (set detection time to 0.8s), and the relative luminous intensity of the previous basic luminous system was 100%, and each group of formulas was calculated respectively The relative luminous intensity and relative activity are shown in Table 2 below:

[0024] Table...

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Abstract

The invention discloses an enhanced acridinium ester luminescence system, which comprises a pre-activating liquid, an activating liquid, and acridinium ester linked to a to-be-detected object. After linked to the to-be-detected object, the acridinium ester linked to the to-be-detected object gives out light through actions of the pre-activating liquid, and the activating liquid in sequence. The pre-activating liquid is a solution of H2O2 containing 0.125-1% of a stabilizer and 0.125-1% of an enhancer, and the activating liquid is a solution of NaOH containing 0.125-1% of the stabilizer and 0.125-1% of the enhancer, wherein the stabilizer is dimethylformamide or ethyl alcohol, and the enhancer is Triton X-100 or Tween-20. According to the invention, the luminescent intensity of the acridinium ester is effectively enhanced, and the probability of occurrence of bubbles in the luminescence system is reduced. The freezing point of the luminescence system is lowered, so that the luminescence system is unlikely to freeze during long-time transportation in winter.

Description

technical field [0001] The invention belongs to the technical field of chemiluminescence immunoassay, in particular to an acridinium ester enhanced luminescence system. Background technique [0002] Immunoassay technology is now widely used in the field of biomedicine, and has become an essential conventional technical means in many fields such as clinical diagnosis, environmental and health testing, forensic identification, food safety, etc., playing an irreplaceable role. Has a huge market. The signal system used by immunoassay reagents plays a decisive role in the performance of immunoassay reagents, from radioimmunoassay (RIA) to enzyme-linked immunosorbent assay (ELISA) to chemiluminescent immunoassay (CLIA), immunoassay technology The development and progress are mainly the progress and revolution of the signaling system. The basis of the immune response using antigens and antibodies and their various conjugates in immunoassay technology has not changed. Therefore, ho...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 翁祖星刘江武魏淑英黄缘缘徐飞海葛胜祥
Owner XIAMEN INNODX BIOTECH CO LTD
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