Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of single hydroxyl phenolic urine metabolite detection reagent

A technology of monohydric phenols and detection reagents, which is applied in the field of preparation of urine monohydric phenols metabolite detection reagents, can solve the problems of complex operation and difficulty in clinical development, and achieve simple operation, low cost, false positive rate and The effect of low false negative rate

Inactive Publication Date: 2015-12-16
SUZHOU RELING BIOTECH CO LTD
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Each method has advantages and disadvantages. For example, the HPLC method for the determination of urinary polyamines has high sensitivity and good selectivity, but the operation is complicated and difficult to carry out clinically.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of single hydroxyl phenolic urine metabolite detection reagent
  • Preparation method of single hydroxyl phenolic urine metabolite detection reagent
  • Preparation method of single hydroxyl phenolic urine metabolite detection reagent

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0022] (1) Prepare ferric sulfate solution as solution A; dissolve 20 g of iron in 2M sulfuric acid solution of 250 ml, and make solution A after iron is completely dissolved

[0023] (2) prepare mercuric sulfate solution, as B solution; 15g mercury is dissolved in the 2M sulfuric acid solution of 250ml, after mercury dissolves completely, make B solution;

[0024] (3) Prepare copper nitrate solution as solution C; dissolve 15 g of copper in 2M nitric acid solution of 250 ml, and prepare solution C after the copper is completely dissolved.

[0025] (4) Prepare ferric nitrate solution as solution D; dissolve 20 g of iron in 250 ml of 2M nitric acid solution, and prepare solution D after the iron is completely dissolved.

[0026] (5) Mix A solution, B solution, C solution, and D solution at a mass ratio of 1:2:1.5:1, add preservatives, stir well and make a light green urine monohydric phenol metabolite detection Reagent;

[0027] The temperature of solution A, solution B, solu...

preparation Embodiment 2

[0029] (1) Prepare ferric sulfate solution as A solution; dissolve 25g of iron in 250ml of 3M sulfuric acid solution, and make A solution after the iron is completely dissolved

[0030] (2) prepare mercuric sulfate solution, as B solution; 20g mercury is dissolved in the 3M sulfuric acid solution of 250ml, after mercury dissolves completely, make B solution;

[0031] (3) Prepare copper nitrate solution as solution C; dissolve 20 g of copper in 2M nitric acid solution of 250 ml, and prepare solution C after the copper is completely dissolved.

[0032] (4) Prepare ferric nitrate solution as solution D; dissolve 20 g of iron in 250 ml of 2M nitric acid solution, and prepare solution D after the iron is completely dissolved.

[0033] (5) Mix A solution, B solution, C solution, and D solution at a mass ratio of 1:1.5:2:1, add preservatives, stir well and make a light green urine monohydric phenol metabolite detection Reagent;

[0034] The temperature of solution A, solution B, so...

preparation Embodiment 3

[0036] (1) Prepare ferric sulfate solution as solution A; dissolve 20 g of iron in 2M sulfuric acid solution of 250 ml, and make solution A after iron is completely dissolved

[0037] (2) prepare mercuric sulfate solution, as B solution; 15g mercury is dissolved in the 2M sulfuric acid solution of 250ml, after mercury dissolves completely, make B solution;

[0038] (3) Prepare copper nitrate solution as solution C; dissolve 25 g of copper in 2M nitric acid solution of 250 ml, and prepare solution C after the copper is completely dissolved.

[0039] (4) Prepare ferric nitrate solution as solution D; dissolve 25g of iron in 2M nitric acid solution of 250ml, and prepare solution D after iron is completely dissolved.

[0040] (5) Mix A solution, B solution, C solution, and D solution at a mass ratio of 1:2:1.5:1, add preservatives, stir well and make a light green urine monohydric phenol metabolite detection Reagent;

[0041] The temperature of solution A, solution B, solution C...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of detection reagents, in particular to a single hydroxyl phenolic urine metabolite detection reagent. The preparation method includes the following steps of preparing 0.2-1g / ml of a ferric sulphate solution serving as a solution A; preparing 0.1-1g / ml of a mercury sulfate solution serving as a solution B; preparing 0.15-1g / ml of a copper nitrate solution serving as a solution C; preparing 0.15-1g / ml of a ferric nitrate solution as a solution D; adding a preservative after mixing the solution A, the solution B, the solution C and the solution D in the mass ratio of 1:(1-2):(1.5-2):1 with uniform stirring to obtain the light-green single hydroxyl phenolic urine metabolite detection reagent, wherein the temperature of the solution A, the solution B, the solution C and the solution C and mixed liquor of the solution A, the solution B, the solution C and the solution D ranges from 30 DEG C to 35DEG C. The detection reagent is prepared from the solution A, the solution B, the solution C and the solution D with the optimal mass ratio and the best mixed concentration, so that the reagent is high in flexibility, low in false positive rate and false negative rate, easy to operate and low in cost.

Description

technical field [0001] The invention relates to the field of detection reagents, in particular to a preparation method of a urine monohydric phenol metabolite detection reagent. Background technique [0002] At present, there are many methods for diagnosing tumors at home and abroad. The common method is to detect various tumor markers such as blood PSA, AFP, CEA, ferritin, CA125 and lipoprotein. Although the detection rate of these markers is high, most of them Both are destructive tests, and the operation is complicated and expensive, so they are not suitable for large-scale population screening. Urine is used to detect tumors, which avoids the link of blood drawing, which is easy to cause infection. There are also many methods at home and abroad, such as: high-performance liquid chromatography for the determination of urinary polyamines, ELISA for the determination of urinary ferritin and the determination of tyrosine in urine Acid and its metabolites and protein chip de...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/82
CPCG01N21/82G01N33/48
Inventor 扈晓佳梅宗泉梅春华
Owner SUZHOU RELING BIOTECH CO LTD