A kind of production method of dendrobium candidum tissue culture bag seedling

Abstract

The invention discloses a production method of tissue culture bag seedlings of Dendrobium officinale, which comprises artificially pollinating different strains of the mother plant of Dendrobium officinale with strong growth, and aseptically sowing the obtained fruits on the seed germination medium when they are basically mature and uncracked. , the seeds germinate into protocorms, and thin the seedlings in separate bottles on the seedling culture medium. After 50-60 days of cultivation, rooted seedlings can be further formed. Transfer the seedlings to film bags, pour liquid strong seedling medium, and seal the bags until they are formed. Robust plants; bagged seedlings are cultivated after 7-14 days of hardening under natural light. The invention has the characteristics of rapid growth of seedlings, good quality, low cost, etc., can obtain a large number of bagged seedlings in a short period of time, and the transplanting survival rate of mature seedlings is over 95%. An effective way can be provided for the production of dendrobium officinale and other seedlings.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for producing dendrobium candidum seedlings by using polypropylene film bags. Background technique [0002] Dendrobium officinale (scientific name: Dendrobium officinale Kimura et Migo), also known as: Black knot grass, Yunnan iron skin. Belonging to the order of Micros, it is a perennial epiphytic herbaceous plant of Orchidaceae. Its stem is used as medicine, and belongs to yin-tonifying medicine in tonic medicine. It has been proven that it has the functions of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, relieving cough and moistening lung, moistening throat and improving eyesight, improving human immune function, preventing tumor and cardiovascular disease, improving sleep, preventing cataract, and prolonging life. However, due to the special growth environment and limited distribution, as well as long-term excavatio...

AI Technical Summary

Problems solved by technology

[0003] Test-tube seedling production has become an important part of modern seedling supply, such as banana seedlings, eucalyptus seedlings, dendrobium seedlings, etc. are mostly produced through test-tube seedling technology; there is a need to use glass tissue culture bottles or plastic tissue culture bottles as culture containers Disadvantages such as cleaning, time-consuming and labor-intensive, and high transportation costs. In recent years, the use of film bags as culture containers has become a research hotspot in test-tube seedling enterprises, and has been promoted in the production of banana seedlings.

Although the existing bag seedling technology can greatly improve sterilization efficiency and reduce cleaning and transportation costs, due to the soft texture of the film bag, it is difficult to form a conventional semi-solid medium, which affects the inoculation operation, so the bag seedling technology is used in the existing seedling production system. The proportion is not very large, especially in the cultivation of Dendrobium officinale, there is still a lot of room for improvement.