A kind of preparation method of drinking water agent for treating bird flu
A drinking water preparation and avian influenza technology, applied in the field of preparation of drinking water preparations, can solve the problems of high storage and stability conditions of vaccines, increase the use cost of vaccines, etc. Effect
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Embodiment 1
[0027] A kind of preparation method of the drinking water preparation for the treatment of bird flu, comprises the steps:
[0028] The first step is to extract the bursa active polypeptide stock solution from the chicken bursa:
[0029] (a) Obtain fresh bursa from healthy adult chickens, remove fascia and fat;
[0030] (b) Add sterile saline to the bursa obtained in step (a) at a mass / volume ratio of g / mL 1:1, and homogenize for 3 min at a speed of 12,000 rpm / min with a tissue masher;
[0031] (c), add sterile saline to the homogenate obtained in step (b) at a volume ratio of 1:6, mix well, and freeze and thaw repeatedly 3 times;
[0032] (d) Under aseptic conditions, add sterile saline to the homogenate after freezing and thawing in step (c) at a volume ratio of 1:3, and use a tissue masher to homogenize again at a speed of 2500 rpm / min for 15 minutes;
[0033] (e) Filter the homogenate obtained in step (d) with 8 layers of sterilized gauze, the filtrate is the stock soluti...
Embodiment 2
[0040] A kind of preparation method of the drinking water preparation for the treatment of bird flu, comprises the steps:
[0041] The first step is to extract the bursa active polypeptide stock solution from the chicken bursa:
[0042] (a) Obtain fresh bursa from healthy adult chickens, remove fascia and fat;
[0043] (b) Add sterile saline to the bursa obtained in step (a) at a mass / volume ratio of g / mL 1:1, and homogenize for 4 min at a speed of 12,000 rpm / min with a tissue masher;
[0044] (c), add sterile saline to the homogenate obtained in step (b) at a volume ratio of 1:6, mix well, and freeze and thaw repeatedly 3 times;
[0045] (d) Under aseptic conditions, add sterile saline to the homogenate after freezing and thawing in step (c) at a volume ratio of 1:3, and use a tissue masher to homogenize again at a speed of 2500 rpm / min for 15 minutes;
[0046] (e) Filter the homogenate obtained in step (d) with 8 layers of sterilized gauze, the filtrate is the stock solution...
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