Macro-element nutrient solution for large-scale culture of Chlorella salina

A seawater chlorella, large-scale cultivation technology, applied in the direction of fertilizer mixture, fertilization device, application, etc., can solve the problems that affect the seedling breeding process and seedling quality, unstable nutrient content of cells, and failure to solve seawater chlorella, etc. Achieve the effect of reducing the cost of breeding, long duration of cultivation, and stable nutritional components

Inactive Publication Date: 2015-12-30
ZHANJIANG HENGXING SOUTHERN OCEAN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

F / 2 medium has disadvantages such as easy contamination and low culture density, which can easily lead to low culture efficiency and unstable nutrient composition of the cultured cells, which seriously affects the seedling cultivation process and quality
Existing technologies fail to address this shortcoming in seawater chlorella cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 adopts the following steps to realize the large-scale cultivation of seawater chlorella:

[0021] (1) Clean and sterilize the used bioreactor, and then introduce seawater that has undergone sand filtration, foam separation, precision filter and ultraviolet disinfection into the cleaned and sterilized bioreactor. The depth of seawater in the reactor is 40cm. Add strong chlorine essence to the seawater according to the dose of 20ppm, disinfect and aerate for a whole day and night;

[0022] (2) Add sodium thiosulfate at a dose of 30ppm to the seawater treated in step (1);

[0023] (3) Nutrient solution preparation: Weigh 10 parts of urea, 1.5 parts of sodium dihydrogen phosphate, 0.45 parts of ferric chloride, 1.41 parts of disodium edetate, and 986.64 parts of water according to the formula, and mix urea, sodium dihydrogen phosphate , ferric chloride, and disodium edetate are dried at a temperature of 50°C and dissolved in water to obtain a nutrient solution...

Embodiment 2

[0026] Embodiment 2 adopts the following steps to realize the large-scale cultivation of seawater chlorella:

[0027] (1) Clean and sterilize the used bioreactor, and then introduce seawater that has undergone sand filtration, foam separation, precision filter and ultraviolet disinfection into the cleaned and sterilized bioreactor. The depth of seawater in the reactor is 40cm. Add strong chlorine essence to the seawater according to the dose of 20ppm, disinfect and aerate for a whole day and night;

[0028] (2) Add sodium thiosulfate at a dose of 30ppm to the seawater treated in step (1);

[0029] (3) Nutrient solution preparation: Weigh 13 parts of urea, 2 parts of sodium dihydrogen phosphate, 0.42 parts of ferric chloride, 1.50 parts of disodium edetate, and 983.08 parts of water according to the formula, and mix urea, sodium dihydrogen phosphate , iron trichloride and disodium edetate are dried at a temperature of 60°C and dissolved in water to obtain a nutrient solution; ...

Embodiment 3

[0032] Embodiment 3 adopts the following steps to realize the large-scale cultivation of seawater chlorella:

[0033] (1) Clean and sterilize the used bioreactor, and then introduce seawater that has undergone sand filtration, foam separation, precision filter and ultraviolet disinfection into the cleaned and sterilized bioreactor. The depth of seawater in the reactor is 40cm. Add strong chlorine essence to the seawater according to the dose of 20ppm, disinfect and aerate for a whole day and night;

[0034] (2) Add sodium thiosulfate at a dose of 30ppm to the seawater treated in step (1);

[0035] (3) Nutrient solution preparation: Weigh 15 parts of urea, 2 parts of sodium dihydrogen phosphate, 0.39 parts of ferric chloride, 1.64 parts of disodium edetate, and 980.97 parts of water according to the formula, and mix urea, dihydrogen phosphate Sodium, ferric chloride, and disodium edetate are dried at a temperature of 65°C and dissolved in water to obtain a nutrient solution; ...

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Abstract

The invention discloses macro-element nutrient solution for large-scale culture of Chlorella salina. The nutrient solution is nutrient solution (1000x) prepared by using 9 to15 parts of urea, 1 to 3 parts of sodium dihydrogen phosphate, 0.3 to 0.5 part of ferric trichloride, 1.3 to 1.8 parts of EDTA-2Na and balance of water. The nutrient solution disclosed by the invention is used for the large-scale culture of Chlorella salina, has the advantages that the growth of Chlorella salina can be promoted, the culture density is improved, the culture efficiency is improved and the cell nutrient components of the obtained Chlorella salina are stable, and further has the characteristics of high culture density, long duration and low cost.

Description

technical field [0001] The invention relates to a microalgae culture nutrient solution, in particular to a macroelement nutrient solution for large-scale cultivation of seawater chlorella, belonging to the field of aquaculture. Background technique [0002] As a renewable biomass energy, microalgae has also received more and more attention. There are also related researches on the industrialization of large-scale cultured microalgae and some progress has been made. However, the existing microalgae industrialization model still has the disadvantages of low efficiency and high cost. [0003] Seawater Chlorella belongs to Chlorophyta, Chlorophyceae, Chlorellaceae. Unicellular algae, often solitary, but also multicellular aggregates. The cells are spherical and oval, with a perigene, cup-shaped or sheet-shaped pigment body inside. Seawater chlorella is a high-quality bait for larvae such as seawater bivalve molluscs and Penaeus vannamei. [0004] At present, the culture of s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 李色东刘涛吴悦
Owner ZHANJIANG HENGXING SOUTHERN OCEAN TECH
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