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Dimethyl phthalate artificial immunogen and preparation and application thereof

A technology of dimethyl phthalate and artificial immunogen, which is applied in the field of dimethyl phthalate artificial immunogen DMP-BSA and its preparation, to achieve the advantages of strong antigen practicability, simple and feasible preparation technology, and low cost Effect

Inactive Publication Date: 2015-12-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no relevant report on the detection of DMP by fluorescent immuno-PCR technology

Method used

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  • Dimethyl phthalate artificial immunogen and preparation and application thereof
  • Dimethyl phthalate artificial immunogen and preparation and application thereof
  • Dimethyl phthalate artificial immunogen and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, the synthesis of dimethyl phthalate hapten

[0054] The synthetic route diagram of dimethyl phthalate hapten is as follows figure 1 Shown:

[0055] (1) Synthesis of dimethyl phthalate hapten intermediate

[0056] Weigh 10g (0.0474mol) of 4-nitrophthalic acid into a round bottom flask, then slowly add 11.5mL (0.2850mol) of methanol, and add 1.65mL (0.0304mol) of concentrated H 2 SO 4 Catalyze, gradually heat up to 90°C and heat under reflux for 5 hours until the raw material point disappears in thin plate chromatography (TLC) detection (chromogenic agent: dissolve a small amount of potassium permanganate in acetone solvent; developing agent, n-hexane: ethyl acetate =5:1); after the reaction, the reaction solution was transferred to a rotary evaporator to distill off unreacted methanol and generated water, then the liquid was poured into ice water while hot, and the solid was precipitated; then the solid crude product was washed with 10% Na 2 CO 3 Th...

Embodiment 2

[0059] Embodiment 2, the synthesis of dimethyl phthalate hapten

[0060] The synthetic route diagram of dimethyl phthalate hapten is as follows figure 1 Shown:

[0061] (1) Synthesis of dimethyl phthalate hapten intermediate

[0062] Weigh 10g (0.0474mol) of 4-nitrophthalic acid into a round bottom flask, then slowly add 9.6mL (0.2370mol) of methanol, and add 1.67mL (0.0308mol) of concentrated H 2 SO 4 Catalyze, gradually warming up to 80°C and heating under reflux for 6 hours until the point of the raw material detected by thin plate chromatography (TLC) disappears (chromogenic agent: a small amount of potassium permanganate dissolved in acetone solvent; developing agent, n-hexane: ethyl acetate =5:1); after the reaction, the reaction solution was transferred to a rotary evaporator to distill off unreacted methanol and generated water, then the liquid was poured into ice water while hot, and the solid was precipitated; then the solid crude product was washed with 10% Na...

Embodiment 3

[0065] Embodiment 3, the synthesis of dimethyl phthalate hapten

[0066] The synthetic route diagram of dimethyl phthalate hapten is as follows figure 1 Shown:

[0067] (1) Synthesis of dimethyl phthalate hapten intermediate

[0068] Weigh 10g (0.0474mol) of 4-nitrophthalic acid into a round bottom flask, then slowly add 12.4mL (0.3081mol) of methanol, and add 1.80mL (0.0332mol) of concentrated H 2 SO 4Catalyze, gradually heat up to 100°C and heat under reflux for 5 hours, until the raw material point detected by thin plate chromatography (TLC) disappears (chromogenic agent: dissolve a small amount of potassium permanganate in acetone solvent; developing agent, n-hexane: ethyl acetate =5:1); after the reaction, the reaction solution was transferred to a rotary evaporator to distill off unreacted methanol and generated water, then the liquid was poured into ice water while hot, and the solid was precipitated; then the solid crude product was washed with 10% Na 2 CO 3 Th...

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Abstract

The invention discloses dimethyl phthalate artificial immunogen and preparation and application thereof. The structural formula of dimethyl phthalate artificial immunogen (DMP-BSA) is shown in the specification. The preparation method of the dimethyl phthalate artificial immunogen comprises the step that the dimethyl phthalate artificial immunogen is prepared by coupling DMP hapten and protein molecules BSA through diazotization. The preparation method of the immunogen is simple, good in stability and low in cost, and industrial production is easy. The DMP-BSA passes through an immune animal to be prepared into a specific antibody, and a foundation is laid for building an immunodetection method.

Description

technical field [0001] The invention relates to a dimethyl phthalate artificial immunogen DMP-BSA and its preparation and application, in particular to a phthalic acid bis-phthalate used in the immunological detection of emerging persistent organic pollutants—plasticizers Methyl ester artificial immunogen DMP-BSA and its preparation method and application. Background technique [0002] Phthalate ester plasticizers (abbreviated as PAEs), also known as phthalates, are a class of Fischer esterification reactions between phthalic acid and alcohols containing 4-15 carbons. A collective term for important derivatives of esters formed. This kind of substance has a special smell, high toxicity, and is usually a viscous liquid; under liquid conditions, it has a wide temperature range, high fluidity, low volatility, insoluble in water, and easily soluble in most organic solvents. Such substances have a wide range of uses, not only as food packaging materials, toys, pesticide carrier...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K1/107G01N33/543
CPCC07K14/765C07K19/00G01N33/543
Inventor 庄惠生孙瑞艳
Owner SHANGHAI JIAO TONG UNIV
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