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Method for preparing armillaria luteo-virens fruiting body anti-hepatoma activity sterol constituent

A technology of Armillaria chrysanthemum spores and sterols, applied in the direction of organic active ingredients, biochemical equipment and methods, medical preparations containing active ingredients, etc., can solve the problems of lack of research on the chemical components of Armillaria chrysanthemum fruiting bodies , to achieve the effects of stable and controllable quality, expanding the source of raw materials, and intuitive results

Inactive Publication Date: 2015-12-30
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the chemical constituents of the fruiting bodies of Armillaria chrysanthemum is also extremely scarce in the literature

Method used

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  • Method for preparing armillaria luteo-virens fruiting body anti-hepatoma activity sterol constituent
  • Method for preparing armillaria luteo-virens fruiting body anti-hepatoma activity sterol constituent
  • Method for preparing armillaria luteo-virens fruiting body anti-hepatoma activity sterol constituent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 A method for preparing the anti-liver cancer active sterol components of Armillaria chrysanthemum fruiting bodies, comprising the following steps:

[0034] (1) 50g of Armillaria chrysanthemum acetone extract, dissolved with methanol at a solid-to-liquid ratio of 1g:50mL, filtered to obtain the filtrate, which was separated on a microporous resin column, and eluted with 100% methanol solution of 3 times the column volume , collected the eluate and dried under reduced pressure to obtain 34.6 g of Armillaria viridosa fruiting body sterols;

[0035] in:

[0036] The conditions for concentration under reduced pressure refer to a vacuum degree of 0.06 MPa and a temperature of 70°C.

[0037] The microporous resin column refers to the MCI type resin column.

[0038] (2) Add 10 times its volume of normal hexane-ethyl acetate mixture to the sterols component of the Armillaria chrysanthemum fruiting body to dissolve, and pass through an organic filter membrane of 0.45 ...

Embodiment 2

[0051] Example 2 A method for preparing the anti-liver cancer active sterol components of Armillaria chrysanthemum fruiting bodies, comprising the following steps:

[0052] (1) Armillaria chrysanthemum acetone extract 100g, dissolve with methanol at a solid-to-liquid ratio of 1g:100mL, filter to obtain the filtrate, separate the filtrate on a microporous resin column, and elute with 90% methanol solution of 5 times the column volume , collect the eluate and dry under reduced pressure to obtain 65.8 g of Armillaria chrysanthemum fruiting body sterols;

[0053] in:

[0054] The conditions for concentration under reduced pressure refer to a vacuum degree of 0.09 MPa and a temperature of 50°C.

[0055] The microporous resin column refers to the MCI type resin column.

[0056] (2) Add 20 times of its volume of n-hexane-ethyl acetate mixed solution to dissolve the Armillaria chrysanthemum fruiting body sterols component, and pass through an organic filter membrane of 0.45 μm after...

Embodiment 3

[0062] Example 3 A method for preparing the anti-liver cancer active sterol components of Armillaria chrysanthemum fruiting bodies, comprising the following steps:

[0063] (1) 200g of Armillaria chrysanthemum acetone extract, dissolved in methanol at a solid-to-liquid ratio of 1g:80mL, filtered to obtain the filtrate, which was separated on a microporous resin column, and eluted with 95% methanol solution of 4 times the column volume , collect the eluate and dry under reduced pressure to obtain 122.8 g of Armillaria chrysanthemum fruiting body sterols;

[0064] in:

[0065] The conditions for concentration under reduced pressure refer to a vacuum degree of 0.08 MPa and a temperature of 60°C.

[0066] The microporous resin column refers to the MCI type resin column.

[0067] (2) Add 3 times of its volume of n-hexane-ethyl acetate mixed solution to dissolve the Armillaria chrysanthemum fruiting body sterols component, and pass through an organic filter membrane of 0.45 μm aft...

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Abstract

The invention discloses a method for preparing an armillaria luteo-virens fruiting body anti-hepatoma activity sterol compound. The method is simple in technology, high in recovery rate, good in repeatability, and stable and controllable in quality. Moreover, a cell growth curve is used as the basic index for judging the influence of standard components and samples on the cell physiological activity such as adhesion to the wall and reproduction. The results are visual and easy to judge. The obtained armillaria luteo-virens fruiting body anti-hepatoma activity constituent is judged to contain ergosterol and 5 alpha-ergosta-7,22-dien-3 beta-ol through further separation, crystallization treatment and NMR carbon spectrum and hydrogen spectrum analysis.

Description

technical field [0001] The invention relates to the technical fields of medicinal chemistry and biomedicine, in particular to an Armillaria chrysanthemum fruiting body anti-liver cancer [0002] Preparation method and application of active sterol components. Background technique [0003] Hepatocellular carcinoma (hepatocellular carcinoma, HCC, hereinafter referred to as liver cancer) is a highly malignant tumor with a dangerous prognosis. The 5-year survival rate is only about 7%, and it is the fifth most common malignant tumor in the world. The highest rate ranks third. The incidence of liver cancer in my country accounts for more than half of the world, and the age of onset tends to be younger. At present, the annual mortality rate of liver cancer in my country is about 20 / 100,000, ranking second in the mortality rate of various malignant tumors in my country, and has been on the rise in the past 10 years. Liver cancer has become a major killer that seriously threatens ...

Claims

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Application Information

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IPC IPC(8): C12Q1/02A61K31/575A61P35/00
Inventor 党军王启兰张莉邵赟梅丽娟陶燕铎史强强
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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