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American cockroach peptide B with anti-hepatoma activity and application of American cockroach peptide B

An anti-liver cancer, cockroach technology, applied in the field of medicine, can solve the problems of hypersensitivity, reduce the efficacy of drugs, can not meet the medical market, etc., and achieve the effect of small dosage

Active Publication Date: 2017-09-08
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These extracts have a great impact on the further preparation of anticancer drugs. For example, impurities in the extracts are likely to cause hypersensitivity, which limits the way of drug use. At the same time, further separation and purification will greatly reduce the efficacy of the drug
Therefore, there are not many studies on the active substances of single components in the American cockroach at present, which is far from meeting the needs of the pharmaceutical market.

Method used

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  • American cockroach peptide B with anti-hepatoma activity and application of American cockroach peptide B
  • American cockroach peptide B with anti-hepatoma activity and application of American cockroach peptide B
  • American cockroach peptide B with anti-hepatoma activity and application of American cockroach peptide B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] American cockroach peptide B exists in the medicinal materials of Periplaneta americana. The extraction method of the American cockroach peptide B comprises the following steps: after the Periplaneta americana medicinal material is extracted with a solvent, then repeatedly extracted and separated by macroporous resin adsorption, dextran gel column chromatography, high performance liquid chromatography, etc., A sample containing cockroach peptide B was obtained.

[0035] Sangon Biology (Shanghai) Co., Ltd. was entrusted to prepare meicockatipe peptide B for pharmacodynamic evaluation test. The physicochemical properties of described melilay peptide B are as follows:

[0036] Number of amino acid residues: 8 1 character: DDLRGDND 3 characters: Asp-Asp-Leu-Arg-Gly-Asp-Asn-Asp Molecular weight: 918.87g / mol Isoelectric point: 3.4 Net charge at pH=7: -3.0 Average Hydrophilicity: 1.7 Hydrophilic residue ratio: 75％

...

Embodiment 2

[0040] 1. Cell culture of human liver cancer HepG2 cell line

[0041] Human liver cancer HepG2 cell line adopts high-glucose DMEM complete medium containing 10% fetal bovine serum, placed in 5% CO 2 , Cultured in a 37°C incubator, and the cells in the logarithmic growth phase were taken for experiments.

[0042] 2. MTT method to detect the survival rate of human liver cancer cell HepG2 cells after treatment

[0043] HepG2 cell seed plate (1×10 5 mL -1, 100 μL) were inoculated in a 96-well plate and cultured for 24 h. Concentrations were configured into cockroach peptide B solutions with concentrations of 6.25, 12.5, 25, 50, 100, and 150 μg / mL, and the above-mentioned 6 concentrations of cockroach peptide B solutions were used for intervention, and 6 replicate wells were set for each concentration. 200 μL, set thalidomide (concentration: 100 μg / mL, 200 μg / mL) positive control group and blank control group at the same time, continue to culture for 24 hours. The absorbance v...

Embodiment 3

[0056] Inhibition of Migration of HepG2 Cells by Peptide B in Cell Scratch Test

[0057] Experimental grouping: blank control group, thalidomide control group (concentration of 100 μg / mL, 200 μg / mL), meicockatin B concentration set to low, medium and high dose groups (25 μg / mL, 50 μg / mL, 100 μg / mL mL).

[0058] HepG2 cells were routinely digested at a density of 2×10 5 mL -1 Inoculate in a 6-well plate, 2 mL per well. After culturing for 24 hours, use a 200 μL pipette tip to draw a straight line at the bottom of the 6-well plate, wash with PBS 2 to 3 times, and add drugs according to the experimental groups. Take pictures of the scratches at 0h and 24h respectively, and measure the width of the scratches. The above experiment was repeated three times, and the average value was taken to calculate the scratch healing rate. Scratch healing rate=(0h scratch width-24h scratch width) / 0h scratch width×100%.

[0059] Data processing adopts SPSS17.0 statistical software and Origi...

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Abstract

The invention provides American cockroach peptide B with anti-hepatoma activity and application of the American cockroach peptide B, belonging to the technical field of medicines. The American cockroach peptide B with the anti-hepatoma activity has an amino acid sequence as shown by a sequence table SEQ ID No.1 in the description. The invention further provides the application of the American cockroach peptide B to prepare medicines for preventing and / or treating hepatoma. The American cockroach peptide B can effectively inhibit the proliferation and migration of hepatoma carcinoma cells, and the inhibitory intensity increases with the increasing of the concentration of the American cockroach peptide B. Compared with other American cockroach extracts, the American cockroach peptide B has an obvious advantage in inhibiting the proliferation of HepG2; and compared with the positive control of thalidomide, the American cockroach peptide B has the same or similar anti-hepatoma effect. The American cockroach peptide B provided by the invention is polypeptide, and can be conveniently and economically produced on a large scale through an artificially synthesized manner, thereby being free from the limit to active material sources and able to meet the market and production requirement.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a cockroach peptide B with anti-liver cancer activity and its application. Background technique [0002] At present, due to environmental pollution and other problems brought about by industrial development, the quality of human living environment is continuously declining, and the incidence and mortality of tumor diseases are also increasing, which seriously threatens people's health and life. The occurrence of cancer is affected by many reasons, such as environmental pollution, chemical pollution, ionizing radiation, free radical toxins or microorganisms and their metabolic toxins and other carcinogens, and may also be affected by the body itself, such as genetic characteristics, endocrine imbalance, immune Functional disorders, etc. can also cause normal cells to become cancerous. According to data released by the International Union Against Cancer, cancer deaths...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K38/08A61P35/00A61P35/04
CPCA61K38/00C07K7/06
Inventor 彭芳王建超王斌李婷袁发璐张蕊何苗张成桂
Owner DALI UNIV
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