Temporary immersion type proliferation and rapid propagation method of orchid protocorm-like body

Abstract

The invention discloses a temporary immersion type proliferation and rapid propagation method of an orchid protocorm-like body. The method includes the following steps of conducting sterilize cultivation on an explant, and conducting induction to obtain the orchid protocorm-like body (PLB); secondly, putting the PLB obtained in the first step in a communicating vessel type temporary plant tissue cultivating device to be cultivated in a temporary immersion mode. The method has the advantages that the cultivation space utilization rate is high, cultivation gas component exchange is facilitated, and therefore the quality and propagation coefficient of culture are increased, and the method is suitable for large-scale production; a cultivation device and accessories can be easily and integrally disinfected and sterilized, full closure is achieved in the cultivation process, and the pollution probability is small; the structure is simple and compact, energy consumption is low, cultivation space is saved, and tissue cultivation cost is reduced; transfer and subculture operation of tissue culture seedlings in the whole tissue cultivation process is reduced, and labor cost and production cost are reduced.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for multiplying and regenerating orchid-like protocorms (Protocrm-likeBody, PLB). Background technique [0002] Plant tissue culture technology has the advantages of large and fast reproduction, easy control of experimental conditions, continuous production, and high production efficiency. It is widely used in crop breeding, germplasm resource preservation, and virus-free seedlings of flowers, fruit trees, vegetables, forest trees, and Chinese herbal medicines. Production and rapid propagation and vegetative propagation, such as the vegetative rapid propagation of lily virus-free seed balls. [0003] Traditional solid or semi-solid tissue culture methods consume a large amount of agar, and a large number of containers need to be handled during filling and cleaning, which consumes a lot of labor and leads to high production costs. [0004] At present, some pla...

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Problems solved by technology

Patent No. CN101768566A discloses a batch-type fermentation and cultivation method of Dendrobium candidum, but the two studies have not introduced and improved the most important intermittent submersion cultivation device, and the size and shape of the device and the placement of plant materials will vary. Proliferation efficiency of explants has a major impact

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