Application of drug containing catechin to preparation of drug for preventing and/or treating leukemia

A catechin, leukemia technology, applied in the field of medicine, to achieve good stability, inhibit cell proliferation, and simplify the storage process

Inactive Publication Date: 2016-01-06
王孝仓
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report in the prior art that catechins can be used to treat leukemia

Method used

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  • Application of drug containing catechin to preparation of drug for preventing and/or treating leukemia
  • Application of drug containing catechin to preparation of drug for preventing and/or treating leukemia
  • Application of drug containing catechin to preparation of drug for preventing and/or treating leukemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Experiment on the therapeutic effect of catechin on leukemia

[0036] 1 test material

[0037] 1.1 Experimental animals

[0038] Balb / c mice, DBA mice, 615 mice, and KM mice are all SPF grade experimental animals, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.

[0039] 1.2 Experimental leukemia cells

[0040] The leukemia cells used in the experiment include: L1210 cells (mouse lymphoid leukemia cells), L615 cells (mouse reticuloid leukemia cells), K562 cells (human chronic myelogenous leukemia cells), HL60 cells (human myelogenous leukemia cells ), the above cell lines were provided by Tianjin Institute of Medical Sciences.

[0041] 1.3 Test samples

[0042] The test sample is CDF (that is, pure catechin) sealed and stored at 4°C. When in use, it is prepared with distilled water at about 35-40°C to make a solution of the required concentration for oral administration to animals, and stored in a refrigerator at 4°C. Drug pre...

Embodiment 2

[0064] Embodiment 2: In vitro inhibition experiment of catechin on leukemia cells:

[0065] 1 Experimental materials

[0066] 1.1 Samples to be tested

[0067] Test sample CDF (i.e. pure catechin), dark brown powder, packed in airtight plastic box, stored in refrigerator at 4°C. When used, it is prepared with 1640 culture medium containing 10% calf serum and diluted in multiples for use in cell experiments, and it is freshly prepared when used. The period of use after the preparation of the medicine is 3 to 4 days.

[0068] 1.2 Leukemia cell lines

[0069] L1210 cells (mouse lymphoid leukemia cells), L615 cells (mouse reticuloid leukemia cells), K562 cells (human chronic myelogenous leukemia cells), HL60 cells (human myeloid leukemia cells), the above leukemia cells were routinely cultured in In 1640 culture medium containing 10% calf serum, subculture once every 2 days, and take the cells in the logarithmic growth phase for experiments.

[0070] 1.3 Instruments and reage...

Embodiment 3

[0091] Embodiment 3: the preparation of catechin capsule

[0092] Prescription: catechin microcrystal 50g, starch 450g, dextrin 200g, magnesium stearate 5g; preparation method: take catechin 50g, add starch, dextrin, mix and sieve, granulate, pass through a 16-mesh sieve , dried at 50 degrees Celsius, granulated, added with 5g of magnesium stearate, mixed evenly, subpackaged into 1000 capsules, and obtained.

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Abstract

The invention provides an application of a drug containing catechin or polymorphic bodies to preparation of a drug for preventing and/or treating leukemia. In vivo experiment research shows that the catechin can significantly inhibit proliferation of related leukemic cells after being orally taken or injected; in vitro experiment research shows that the catechin can inhibit growth of leukemic cells. The catechin has wider treatment range for leukemia and has exact treatment effects on leukemia including lymphocytic leukemia, reticulocyte leukemia, chromic myeloid leukemia and the like. Meanwhile, toxic and side effects are not discovered, so that the drug has prominent promotion prospect.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the use of medicines including catechins in the preparation of medicines for preventing and / or treating leukemia. Background technique [0002] Leukemia is a kind of malignant clonal disease of hematopoietic stem cells. Due to uncontrolled proliferation, differentiation disorder, apoptosis inhibition and other factors, leukemia cells are malignant and unlimitedly proliferate in bone marrow and other hematopoietic tissues, thereby infiltrating other tissues and organs, affecting normal hematopoiesis Function, with the development of the course of the disease will bring a series of symptoms, until life-threatening. Clinically, leukemia is characterized by fever, hemorrhage, anemia, and enlargement of liver, spleen, and lymph nodes; it is divided into acute and chronic according to the natural course of the disease and the degree of immaturity of cells; it is divided into granulo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/353A61P35/02
Inventor 王孝仓
Owner 王孝仓
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