Pharmaceutical composition for restraining cerebral thrombosis
A technology of cerebral thrombosis and composition, which is applied in the field of pharmaceutical composition for inhibiting cerebral thrombosis, and achieves the effect of definite curative effect and low toxicity and side effects
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Embodiment 1
[0015] Embodiment 1 is to the preparation of sitting leaf water extract
[0016] Put 800 g of the dry product of sitting leaves into a round-bottomed flask, and successively use 8 L of 95% (v / v) ethanol and 8 L of 75% (v / v) ethanol to extract twice, each time for 4 hours, pour out the ethanol extract, Add 10L of water to the obtained dregs, decoct twice for 2 hours each time, combine the two water extracts, filter, concentrate into a thick paste, dry under reduced pressure at 70°C, pulverize into fine powder, and pass through a 100-mesh sieve to obtain the opposite leaf water extract.
Embodiment 2
[0017] Embodiment 2 is to the preparation of sitting leaf water extract
[0018] Put 1 kg of the dry product of sitting leaves into a round bottom flask, and successively use 10 L of 90% (v / v) ethanol and 10 L of 70% (v / v) ethanol to extract twice, each time for 4 hours, and pour out the ethanol extract. Add 12L of water to the obtained dregs, decoct twice for 2 hours each time, combine the two water extracts, filter, concentrate into a thick paste, dry under reduced pressure at 70°C, pulverize into fine powder, pass through a 100-mesh sieve, and obtain the opposite leaf water extract.
Embodiment 3
[0021] Example 3 Study on the Inhibition of Cerebral Thrombosis Mice by the Extract of Siscifolia
[0022] 36 healthy SD rats, half male and half female, body weight 200-220g, rats were randomly and evenly divided into the following three groups according to body weight and sex: ① model control group, intragastric administration of normal saline 1ml / 100g body weight; ② alcohol extraction Object group, gavage of 42 mg / kg of the ethanol extract of citrate leaf prepared by the process of comparative example; 3. water extract group, gavage of 42 mg / kg of citrate leaf water extract prepared by the process of embodiment 1 of the present invention. Rats in each group were given the corresponding test substance once a day for 8 consecutive days.
[0023] One hour after the last administration, the cerebral thrombosis model was prepared as follows: rats were anesthetized by intraperitoneal injection of 3% pentobarbital sodium 30 mg / kg. Separate the trachea and perform tracheal intubat...
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