Preparation and application of imprinted electrochemical sensor modified by carbon dot composite material

A composite material and sensor technology, which is applied in the detection of sunset yellow in food, and in the field of sunset yellow detection by imprinted electrochemical method, can solve the problems of cumbersome operation, inappropriateness, and expensive equipment, and achieve good selectivity, good conductivity, Detect fast effects

Inactive Publication Date: 2016-01-06
UNIV OF JINAN
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  • Application Information

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Problems solved by technology

The current sunset yellow detection methods mainly include thin-layer chromatography, spectrophotometry, high-performance liquid chromatography, etc. These

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of graphene / magnetic carbon dots / gold nanoparticles composites (GO / MCD / AuNPs)

[0026] 5.0g chitosan and 5.0g nano-ferric oxide were uniformly dispersed in acetic acid solution by ultrasonic, and then transferred to an autoclave for 12 hours at 180°C to obtain magnetic carbon dots (MCD); 0.1g graphene and 0.1g The magnetic carbon dots are ultrasonically dispersed in ultrapure water, add 5mL polydiallyldimethylammonium chloride (PDDA) with a mass fraction of 20%, and stir mechanically for 12h; then add 3.65mL0.01mol / L chloroauric acid solution , heated to a slight boil, then quickly added 3.0mL of 1% sodium citrate solution, and stirred for 30min. The GO / MCD / AuNPs composite was obtained by magnetic cleaning and separation for more than 3 times, and dried.

Embodiment 2

[0028] Preparation of graphene / magnetic carbon dots / gold nanoparticles composites (GO / MCD / AuNPs)

[0029] 5.0g chitosan and 5.0g nanometer iron ferric oxide were uniformly dispersed in acetic acid solution by ultrasonic, and then transferred to an autoclave for 12 hours at 200°C to obtain magnetic carbon dots (MCD); 0.1g graphene and 0.1g The magnetic carbon dots are ultrasonically dispersed in ultrapure water, add 7mL polydiallyldimethylammonium chloride (PDDA) with a mass fraction of 20%, and stir mechanically for 10h; then add 3.65mL0.01mol / L chloroauric acid solution , heated to a slight boil, then quickly added 3.0mL of 1% sodium citrate solution, and stirred for 30min. The GO / MCD / AuNPs composite was obtained by magnetic cleaning and separation for more than 3 times, and dried.

Embodiment 3

[0031] A method for preparing a molecularly imprinted electrochemical sensor for detecting sunset yellow, the steps are as follows:

[0032] (1) Drop 7 μL GO / MCD / AuNPs homogeneous solution onto the surface of the glassy carbon electrode and dry at room temperature;

[0033] (2) Prepare a 0.1mol / L KCl solution, add an appropriate amount of template molecules (sunset yellow) and functional monomers (pyrrole), and complete the pre-assembly process of template molecules and functional monomers at room temperature for 2 hours; use electropolymerization to polymerize MIPs in (1) The obtained GO / MCD / AuNPs / GCE surface was dried at room temperature;

[0034] (3) Elute with methanol-acetic acid mixed solution for 10 times to remove the sunset yellow template molecules on the surface of the sensor, and dry at room temperature;

[0035] (4) With Ag / AgCl as the reference electrode and the Pt electrode as the counter electrode, the sensor obtained in (3) combined with the template molecule...

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Abstract

The invention discloses a molecularly-imprinted electrochemical sensor for detecting sunset yellow. According to the imprinted electrochemical sensor, through the electrode surface modification technology, the surface of a glassy carbon electrode is covered with prepared oxidized graphene/magnetic carbon dots/gold nano particle compounds, and then the purposes of enhancing electric signals and improving the detection sensitivity of the sensor are achieved. By means of an electric polymerization mode, molecularly-imprinted polymers with pyrrole serving as a functional monomer and sunset yellow serving as template molecules are modified to the surface of the electrode, after elution of the template molecules, holes are formed in the surface of the sensor, and the holes and the structure of sunset yellow can achieve specificity matching. The successfully-prepared electrochemical sensor is a working electrode, through specific binding of the template molecules on the surface of the electrode and the holes, the current change is generated, and specificity detection of sunset yellow can be achieved through the current change. The prepared molecularly-imprinted electrochemical sensor is high in selectivity, high in sensitivity, easy and quick to operate and suitable for detection of sunset yellow in food.

Description

technical field [0001] The invention belongs to the field of nanomaterials and molecularly imprinted electrochemical sensors, and particularly relates to the detection of sunset yellow by an imprinted electrochemical method modified by a carbon dot composite material, which can be used for the detection of sunset yellow in food. Background technique [0002] Sunset yellow is a kind of azo dye, which is widely used in beverage, candy, cream products and other food industries. Appropriate addition of sunset yellow can not only change the appearance of food, but also maintain the color of food itself during food processing and storage. However, excessive intake of sunset yellow can cause damage to liver cells, and in severe cases, it can cause kidney failure. Therefore, it is necessary to establish a simple, sensitive, economical and rapid method for the detection of sunset yellow. The current sunset yellow detection methods mainly include thin-layer chromatography, spectroph...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
Inventor 王晓娇罗川南段辉敏李剑波王延辉孙敏冯娟娟
Owner UNIV OF JINAN
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