Hydroponic Propagation Method of Fang's Begonia
A technology of Fang's begonia and hydroponics is applied in the field of hydroponic reproduction of Fang's begonia, which can solve the problems of long germination time, slow rooting, and root cutting, and achieve the effects of promoting robust growth, increasing germination rate and improving survival rate.
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Embodiment 1
[0018] (1) On June 10, 2015, select healthy and mature leaves, disinfect them with 70% alcohol by volume, and cut the petiole short so that each leaf retains a small petiole or total petiole with a length of 0.5-1.0 cm;
[0019] (2) Spread a layer of 0.3-0.5cm thick absorbent cotton in a sterile petri dish, and spread a layer of sterile qualitative filter paper on it; put the leaves flat on the sterile qualitative filter paper, soak the absorbent cotton with the following rooting culture solution And filter paper: sterile water + 10mg / L vitamin B1 + 10mg / L rooting powder (ABT) solution, and make sure that no water accumulates on the surface of sterile qualitative filter paper; carry out rooting culture under the conditions of temperature 23 ℃ and light intensity 3000lux , cultivated in light for 10 hours a day, cultured in dark for 14 hours, and supplemented the above rooting culture solution every 2 to 3 days, controlled the culture solution fluid within 1mL, kept absorbent co...
Embodiment 2
[0024] (1) Select healthy and mature mature leaves, disinfect with 70% alcohol by volume, and cut short petioles so that each leaf retains a small petiole or total petiole with a length of 0.5-1.0 cm;
[0025] (2) Spread a layer of 0.3-0.5cm thick absorbent cotton in a sterile petri dish, and spread a layer of sterile qualitative filter paper on it; put the leaves flat on the sterile qualitative filter paper, soak the absorbent cotton with the following rooting culture solution And filter paper: 30mg / L vitamin B1+1mg / L NAA+0.3mg / L KT, and make the surface of the sterile qualitative filter paper free of water; carry out rooting culture under the conditions of temperature 23 ℃ and light intensity 5000lux, light every day Cultivate for 10 hours, culture in dark for 14 hours, and replenish the above-mentioned rooting culture solution every 2 to 3 days, control the liquid accumulation of the culture solution to less than 1mL, keep the absorbent cotton and filter paper moist, and gro...
Embodiment 3
[0030] (1) On June 26, 2015, select healthy and mature mature leaves, disinfect them with alcohol with a volume concentration of 70%, and cut the petiole short so that each leaf retains a small petiole or total petiole with a length of 0.5-1.0 cm;
[0031] (2) Spread a layer of 0.3-0.5cm thick absorbent cotton in a sterile petri dish, and spread a layer of sterile qualitative filter paper on it; put the leaves flat on the sterile qualitative filter paper, soak the absorbent cotton with the following rooting culture solution And filter paper: sterile water + 30mg / L vitamin B1 + 20mg / L rooting powder (ABT) solution, and make the surface of the sterile qualitative filter paper free of water; carry out rooting culture at a temperature of 24°C and a light intensity of 4000lux , cultivated in light for 10 hours a day, cultured in dark for 14 hours, and replenished the above rooting culture solution every 2 to 3 days, controlled the culture fluid fluid within 1mL, and kept the absorbe...
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