Hydroponic Propagation Method of Fang's Begonia

A technology of Fang's begonia and hydroponics is applied in the field of hydroponic reproduction of Fang's begonia, which can solve the problems of long germination time, slow rooting, and root cutting, and achieve the effects of promoting robust growth, increasing germination rate and improving survival rate.

Active Publication Date: 2018-05-04
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, distilled water or sterile water can be used as the culture medium, and the cut leaves or leaves with petioles are used as the hydroponic propagation method in the absorbent cotton culture dish. The cut leaves or petioles take root slowly, 20 to 25 days, and the number of roots is small, 2 ~3, slender; no germination after rooting, even if it germinates, it takes 30-35 days for a long time to germinate, the number of buds is small, only 3-5, and the germination rate is low, only 30-40%; when transplanting, the root Extending into the absorbent cotton is easy to cause root breakage. Use peat or garden soil as the cultivation substrate. The soil is prone to water accumulation or hardening, causing root rot, and the adventitious root has weak water absorption capacity, photosynthesis is not strong, the survival rate is 60-70%, and the reproduction cycle is long. 60~65 days

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) On June 10, 2015, select healthy and mature leaves, disinfect them with 70% alcohol by volume, and cut the petiole short so that each leaf retains a small petiole or total petiole with a length of 0.5-1.0 cm;

[0019] (2) Spread a layer of 0.3-0.5cm thick absorbent cotton in a sterile petri dish, and spread a layer of sterile qualitative filter paper on it; put the leaves flat on the sterile qualitative filter paper, soak the absorbent cotton with the following rooting culture solution And filter paper: sterile water + 10mg / L vitamin B1 + 10mg / L rooting powder (ABT) solution, and make sure that no water accumulates on the surface of sterile qualitative filter paper; carry out rooting culture under the conditions of temperature 23 ℃ and light intensity 3000lux , cultivated in light for 10 hours a day, cultured in dark for 14 hours, and supplemented the above rooting culture solution every 2 to 3 days, controlled the culture solution fluid within 1mL, kept absorbent co...

Embodiment 2

[0024] (1) Select healthy and mature mature leaves, disinfect with 70% alcohol by volume, and cut short petioles so that each leaf retains a small petiole or total petiole with a length of 0.5-1.0 cm;

[0025] (2) Spread a layer of 0.3-0.5cm thick absorbent cotton in a sterile petri dish, and spread a layer of sterile qualitative filter paper on it; put the leaves flat on the sterile qualitative filter paper, soak the absorbent cotton with the following rooting culture solution And filter paper: 30mg / L vitamin B1+1mg / L NAA+0.3mg / L KT, and make the surface of the sterile qualitative filter paper free of water; carry out rooting culture under the conditions of temperature 23 ℃ and light intensity 5000lux, light every day Cultivate for 10 hours, culture in dark for 14 hours, and replenish the above-mentioned rooting culture solution every 2 to 3 days, control the liquid accumulation of the culture solution to less than 1mL, keep the absorbent cotton and filter paper moist, and gro...

Embodiment 3

[0030] (1) On June 26, 2015, select healthy and mature mature leaves, disinfect them with alcohol with a volume concentration of 70%, and cut the petiole short so that each leaf retains a small petiole or total petiole with a length of 0.5-1.0 cm;

[0031] (2) Spread a layer of 0.3-0.5cm thick absorbent cotton in a sterile petri dish, and spread a layer of sterile qualitative filter paper on it; put the leaves flat on the sterile qualitative filter paper, soak the absorbent cotton with the following rooting culture solution And filter paper: sterile water + 30mg / L vitamin B1 + 20mg / L rooting powder (ABT) solution, and make the surface of the sterile qualitative filter paper free of water; carry out rooting culture at a temperature of 24°C and a light intensity of 4000lux , cultivated in light for 10 hours a day, cultured in dark for 14 hours, and replenished the above rooting culture solution every 2 to 3 days, controlled the culture fluid fluid within 1mL, and kept the absorbe...

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PUM

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Abstract

The invention discloses a hydroponic propagation method of Begonia fangshii. The mature leaves are sterilized, a layer of absorbent cotton is spread in a sterile culture dish, and a layer of sterile qualitative filter paper is laid on it; the leaves are placed flat on the sterile qualitative filter paper, Soak absorbent cotton and filter paper with rooting culture solution for rooting culture until 4 to 7 adventitious roots grow from the petiole; add adventitious bud induction solution to moisten absorbent cotton and filter paper for rooting culture until 5 to 10 buds germinate at the root, supplement without The 1 / 2 MS growth medium prepared with bacterial water continued to be cultivated until the germinated buds developed and grew small leaves; the leaves were sterilized in the shade shed, planted in the substrate, and sprayed into the shade shed to increase the air humidity to obtain begonia seedlings. The begonia Fangshi bred by the method has a survival rate of 99-100%, a germination rate increased by 40%, a survival rate increased by 30%, and a period shortened by nearly one time, which has popularization significance in begonia species conservation and production.

Description

technical field [0001] The invention relates to the technical field of plant asexual propagation, in particular to a hydroponic propagation method of Fang's begonias. Background technique [0002] Fang's Begonia ( Begonia fangii ) compound leaves, leathery leaves, red veins, with high ornamental value. At present, distilled water or sterile water can be used as the culture medium, and the cut leaves or leaves with petioles are used as the hydroponic propagation method in the absorbent cotton culture dish. The cut leaves or petioles take root slowly, 20 to 25 days, and the number of roots is small, 2 ~3, slender; no germination after rooting, even if it germinates, it takes 30-35 days for a long time to germinate, the number of buds is small, only 3-5, and the germination rate is low, only 30-40%; when transplanting, the root Extending into the absorbent cotton is easy to cause root breakage. Use peat or garden soil as the cultivation substrate. The soil is prone to water a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00
CPCA01G24/00A01G31/00
Inventor 杜文文崔光芬王祥宁王继华李绅崇段青贾文杰马璐琳李涵
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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