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Application of triterpene glucoside or pharmaceutically acceptable salts thereof in preparation of tumor radiosensitizer

A technology of mogroside saponin and radiosensitizer, applied in the application field of mogroside saponin, to achieve the effect of improving radiosensitivity

Active Publication Date: 2016-01-13
BEIJING UNIV OF AGRI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Second, radiotherapy alone is effective only for radiation-sensitive malignant tumors
It is used as an active ingredient in the preparation of tumor radiosensitizers, but no related records have been seen so far

Method used

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  • Application of triterpene glucoside or pharmaceutically acceptable salts thereof in preparation of tumor radiosensitizer
  • Application of triterpene glucoside or pharmaceutically acceptable salts thereof in preparation of tumor radiosensitizer
  • Application of triterpene glucoside or pharmaceutically acceptable salts thereof in preparation of tumor radiosensitizer

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0064] This experiment verifies that the CASnumber is 88901-38-6 and the molecular formula is C 42 h 72 o 14 The radiosensitizing effect of mogroside Ⅱ on liver cancer cells.

[0065] 1. WesternBlot method (ie WB method) was used to detect the effect of mogroside Ⅱ on P53 and Bcl-2 in liver cancer cells Hep-G2

[0066] Hep-G2 cells were treated with different concentrations of drugs (0 μmol·L -1 , 10 μmol L -1 , 30 μmol L -1 , 60 μmol L -1 ) was cultured for 24 hours, the cell culture was terminated, and the culture medium was sucked off, washed with PBS (concentration: 0.01 molL-1, pH 7.4), added 50 μL / well of lysate containing PMSF, and placed in an ice bath environment for lysis for 30 min. After 14000rmin -1 Centrifuge for 10 min at a high speed to obtain total protein. The protein concentration was measured by BCA colorimetry, 50 μg of total protein was taken, separated by 12% SDS polyacrylamide gel electrophoresis, electrotransferred to PVDF membrane, blocked wit...

Embodiment 2

[0083] This experiment verifies that the CASnumber is 88901-36-4 and the molecular formula is C 60 h 102 o 29 The radiosensitizing effect of mogroside V on lung cancer cells.

[0084] 1. Effect of mogroside V on P53 and Bcl-2 in lung cancer cells

[0085] The effect of mogroside Ⅴ on P53 and Bcl-2 was detected by protein immunoassay WesternBlot.

[0086] Hep-G2 cells were treated with different concentrations of drugs (0 μmol·L -1 , 10 μmol L -1 , 30 μmol L -1 , 60 μmol L -1 ) was cultured for 24 hours, the cell culture was terminated, and the culture medium was sucked off, washed with PBS (concentration: 0.01 molL-1, pH 7.4), added 50 μL / well of lysate containing PMSF, and placed in an ice bath environment for lysis for 30 min. After 14000rmin -1 Centrifuge for 10 min at a high speed to obtain total protein. BCA colorimetric method was used to measure protein concentration, 50 μg total protein was taken, separated by 12% SDS polyacrylamide gel electrophoresis, electr...

Embodiment 3

[0103] This experimental example verifies the CASnumber89590-98-7, the molecular formula is C 66 h 112 o 34 Radiation-sensitizing effect of mogroside Ⅵ on cervical cancer Hela cells.

[0104] clone forming experiment

[0105] Cellular radiosensitivity was measured by colony formation assay. Dilute Hela cells to 1 x 10 4 / mL, added to 96-well plate, 100 μL per well. Divided into pure irradiation group, combined group (drug + irradiation group). Before irradiation, mogroside Ⅵ (concentration of 10 μmol / L in the combination group) was added to the treatment in the drug+irradiation group for 24 hours. Then, at room temperature, use 6MV-X-ray single irradiation, the doses are 0Gy, 2Gy, 4Gy, 6Gy, 8Gy respectively, irradiation conditions: 6MV-X-ray, room temperature irradiation, irradiation field area 15cm×15cm, plus 1.5cm, etc. Effective tissue filler. After irradiation, replace the culture medium and continue to incubate for 2 weeks, discard the supernatant of each well, fi...

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Abstract

The invention provides novel application of triterpene glucoside or pharmaceutically acceptable salts thereof. The triterpene glucoside or pharmaceutically acceptable salts thereof can be used for preparing tumor radiosensitizer. When the tumor radiosensitizer prepared from the triterpene glucoside and the pharmaceutically acceptable salts thereof which serve as active ingredients is used for tumor radiotherapy, radiosensitivity of tumors can be improved, and side effects of radiotherapy can also be reduced. In addition, the triterpene glucoside is non-toxic and does not hurt human normal cells, and therefore, the application of the triterpene glucoside or pharmaceutically acceptable salts thereof has a clinical application prospect.

Description

technical field [0001] The present invention relates to the field of application of mogroside, in particular to the application of mogroside or a pharmaceutically acceptable salt thereof in the preparation of a tumor radiosensitizer. Background technique [0002] Tumors include malignant tumors and benign tumors. Benign tumors can generally be cured by surgical resection and are less harmful to the human body. However, malignant tumors are more harmful to the human body. At present, malignant tumors have become a disease with a high incidence and a high mortality rate in our country. At present, radiotherapy, chemotherapy, and surgical resection are the main methods for the treatment of malignant tumors. After more than a hundred years of development, radiation therapy has become a mature and effective treatment for tumor diseases. More than half of the malignant tumor patients need radiation therapy at different stages of the disease. [0003] Although the curative effec...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61K41/00A61P35/00
CPCA61K31/704A61K36/42A61K41/0038A61P35/00
Inventor 刘灿马兰青窦德泉荣龙赵晓萌常明明张婧刘续航孙媛霞
Owner BEIJING UNIV OF AGRI
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