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A method for preparing anti-tumor compound eupenifeldin and its special strain

A technology of compound and Phoma, applied in the field of biomedicine, can solve the problems of no researchers, in-depth study of anti-cancer mechanism, no one can successfully research and develop, etc., and achieve the effect of simple operation and low cost

Active Publication Date: 2018-05-25
INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the compound Eupenifeldin has a potential anticancer effect, but so far no one has been able to successfully research and develop this compound, and no researchers have further conducted in-depth research on the anticancer mechanism.

Method used

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  • A method for preparing anti-tumor compound eupenifeldin and its special strain
  • A method for preparing anti-tumor compound eupenifeldin and its special strain
  • A method for preparing anti-tumor compound eupenifeldin and its special strain

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Example 1, Isolation and Identification of Phoma sp. XZ068

[0050] 1. Isolation of strains

[0051] Separation method: Take soil samples from the Qinghai-Tibet Plateau in Tibet, fully mix the preserved soil samples, weigh 10g, add it to a triangular flask filled with 90mL sterilized water, and place the triangular flask on a shaker at 110-130 rpm , and shake for 20-25 minutes to disperse the soil particles evenly in distilled water to obtain a soil suspension with a dilution factor of 10; draw 1 mL from it and put it into a test tube filled with 9 mL of sterilized water to obtain a suspension with a dilution factor of 100. When the sterilized 1 / 4 PDA medium (PotatoDextrose Agar Medium, potato dextrose agar medium) is cooled to about 60°C, add 100 μg / mL streptomycin and 100 μg / mL chloramphenicol, pour it into a petri dish to solidify and cool down, Fully shake the obtained soil suspension with a dilution factor of 100, draw 0.1mL, drop it into a petri dish, spread it e...

Embodiment 2

[0073] Embodiment 2, utilize Phoma sp. (Phoma sp.) XZ068 to prepare compound Eupenifeldin

[0074] 1. Solid fermentation of Phoma sp. XZ068

[0075] 1. Cell activation

[0076] The mycelium of Phoma sp. XZ068CGMCC No.10481 obtained in Example 1 was picked and inoculated on a PDA plate, and cultured at 25° C. for 5 days.

[0077] Wherein, the composition of PDA culture medium is as follows, contains potato 200g (use after boiling juice), glucose 20g, agar 15g in every liter of culture medium, surplus is distilled water. The medium was sterilized by high-pressure steam at 121°C for 30 minutes before use.

[0078] 2. Seed cultivation

[0079] Method: After the bacteria overgrow the plate, put five 1cm 2 Step 1 of the size Activated Phoma sp. XZ068 bacterium blocks were inoculated into a 250mL Erlenmeyer flask containing 50mL seed medium together with the culture medium, and cultivated on a shaker for 5 days (cultivation temperature: 25°C; rotation speed: 200rpm), to obtain t...

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Abstract

The invention discloses a method for preparing the anti-tumor compound Eupenifeldin and its special bacterial strain. The special bacterial strain for preparing the anti-tumor compound Eupenifeldin provided by the present invention is specifically Phoma sp. XZ068, and its preservation number in the General Microbiology Center of China Committee for the Collection of Microorganisms is CGMCC No. 10481. Experiments have proved that the present invention utilizes Phoma sp. (Phoma sp.) XZ068CGMCC No.10481 to prepare the method for the antitumor active compound Eupenifeldin, and the yield is high (the crude extract of 10.3g ethyl acetate obtains 400mg pure compound Eupenifeldin through separation and purification, The purity is more than 99%, and the yield is 3.9%), and the method of the invention is simple to operate, low in cost, time-saving and labor-saving.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a method for preparing an antitumor compound Eupenifeldin and a special bacterial strain thereof. Background technique [0002] Malignant tumors are major malignant diseases that threaten human health and life. According to the "World Cancer Report" published by the World Health Organization in 2014, there were 14 million new cancer cases worldwide in 2012, of which 8.2 million died. At the same time, it is predicted that the number of cancer cases in the world will increase rapidly from 14 million in 2012 to 19 million in 2025 and 24 million in 2035, and the number of cancer deaths will also soar from 8.2 million per year in the same period to 13 million. Cancer is becoming the number one killer of human beings. After years of hard work by scientists from various countries, new anti-tumor drugs have emerged continuously, and the chemical drug treatment of tumors has developed rapidly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P17/06C12R1/645
Inventor 车永胜张晋瑜张杨王勃
Owner INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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