Preparation method of raspberry induction culture medium

A technology of induction medium and preparation method, which is applied in the field of preparation of raspberry induction medium, can solve the problems of production decline, virus accumulation, slow reproduction speed, etc., and achieves improved yield and quality, simple and easy-to-obtain components, and improved induction rate effect

Inactive Publication Date: 2016-01-20
钟煜
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  • Application Information

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Problems solved by technology

Artificial cultivation generally adopts asexual reproduction methods such as cuttings, layering, and divisions. The reproduction speed is slow, and the production of seedlings cannot meet the needs of large-scale production. Moreover, long-term asexual reproduction will cause virus accumulation, aggravate the damage, decrease yield, and deteriorate quality, which affects farmers. income

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  • Preparation method of raspberry induction culture medium
  • Preparation method of raspberry induction culture medium

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Embodiment Construction

[0014] The invention relates to a preparation method of raspberry induction medium, which is characterized in that it comprises distilled water, basic MS dry powder medium, naphthalene acetic acid NAA is 0.1-0.3mg / L, 6-benzylaminoadenine MS+6-BA is 1.5 -2.5mg / L, acid-base blending agent, the described preparation method comprises the steps:

[0015] (1) Add 500ml of distilled water and basic MS dry powder medium into the enamel container, heat and stir until completely mixed;

[0016] (2) Continue to heat the medium mixture, slowly add naphthaleneacetic acid NAA 0.1-0.3 mg, 6-benzylaminoadenine MS+6-BA 1.5-2.5 mg and keep stirring until completely mixed;

[0017] (3) Add distilled water to the enamel container and dissolve to 1L to obtain α medium;

[0018] (4) Adjust the pH value of the α medium to 5.8 with an acid-base regulator;

[0019] (5) For filling, take 45ml of α-medium in each glass bottle and tighten the bottle cap;

[0020] (6) Put the filled α-medium into a ste...

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Abstract

The invention provides a preparation method of a raspberry induction culture medium. The method is characterized by comprising distilled water, basic MS dry powder culture medium, 0.1 to 0.3mg / L of NAA (naphthalene acetic acid), 1.5 to 2.5mg / L of MS+6-BA (6-benzylamino adenine) and acid and alkali blenders. The preparation method comprises the following steps of (1) adding 500ml of distilled water and basic MS dry powder culture medium into an enameled container; heating is performed; the materials are continuously stirred until the materials are completely mixed; (2) the culture medium mixture is continuously heated, and 0.1 to 0.3mg of NAA and 1.5 to 2.5mg of MS+6-BA are slowly added and are continuously stirred until the materials are completely mixed; (3) the distilled water is added into the enameled vessel for realizing the constant volume to 1L, and an alpha culture medium is obtained; (4) the acid and alkali blenders are used for regulating the PH values of the alpha culture medium into 5.8; (5) filling is carried out; (6) the filled alpha culture medium is put into a disinfection sterilization pot to be sterilized, and the sterilization is performed for 20 minutes; (7) the alpha culture medium is taken out to be flatly put on a test bed and is cooled and condensed into a gel state.

Description

technical field [0001] The invention relates to a preparation method of a raspberry induction medium, which belongs to the technical field of plant tissue culture. Background technique [0002] At present, raspberries are mainly wild, and the distribution of resources is relatively scattered, no one manages them, and the output is extremely low. Artificial cultivation generally adopts asexual reproduction methods such as cuttings, layering, and divisions. The reproduction speed is slow, and the production of seedlings cannot meet the needs of large-scale production. Moreover, long-term asexual reproduction will cause virus accumulation, aggravate the damage, decrease yield, and deteriorate quality, which affects farmers. income. Contents of the invention [0003] The purpose of the present invention is in order to overcome the shortcoming that prior art exists, provide a kind of preparation method that promotes raspberry growth, effectively improves the induction rate of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 钟煜范灵丹余敏佳林昱张婷吕康
Owner 钟煜
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