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Application of ALDH2 genes to preparation of medicine for preventing genetic toxicity caused by styrene

A genotoxic, styrenic technique for the function and application of genes

Inactive Publication Date: 2016-01-20
FUZHOU UNIV
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Problems solved by technology

Therefore, the focus of this study is on the metabolic enzymes related to styrene, especially whether the acetaldehyde dehydrogenase (ALDH2) gene has an effect on the genotoxicity caused by styrene, so far there is no relevant report

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  • Application of ALDH2 genes to preparation of medicine for preventing genetic toxicity caused by styrene
  • Application of ALDH2 genes to preparation of medicine for preventing genetic toxicity caused by styrene
  • Application of ALDH2 genes to preparation of medicine for preventing genetic toxicity caused by styrene

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Embodiment Construction

[0014] The process of the present invention will be further described in detail below, but the embodiment of the present invention is not limited thereto.

[0015] Animal experiment:

[0016] Species, gender, age and source of experimental animals: the experimental subjects were mice of C57BL / 6J background, including male and female, aged 8 weeks, weighing between 19-24g. Aldh2 knockout and wild-type mice were kindly provided by the National Institute of Labor Safety and Health, Japan.

[0017] Male and female mice of KO type and WT type were divided into 6 mice / group. Inhalation exposure experiments were carried out in specific stainless steel cages, and the exposure concentrations of styrene were 0, 20, 100, and 200 ppm. During the exposure, the styrene concentration was monitored by gas chromatography (Shimadzu GC-7A, Kyoto, Japan). A control group was exposed to filtered air. Mice were exposed for 6 hours per day, continuously for 5 days / week, for a total of 6 weeks. ...

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Abstract

The invention discloses application of ALDH2 genes to preparation of medicine for preventing genetic toxicity caused by styrene and belongs to the field of functions and application of genes. According to the application, an Aldh2 gene knockout type and wild type mouse is used as a model; after the mouse is exposed in styrene by inhaling styrene, the concentrations of mandelic acid and phenylglyoxylic acid, final metabolites of styrene, in the mouse are detected, and multiple biological indexes, representing genetic toxicity, of DNA basic damage, oxidative damage and 8-hydroxyl deoxyguanosine (8-OH-dG) are used for quantization of genetic toxicity caused by styrene. Results clearly show that the ALDH2 genes have an adjusting effect on genetic toxicity caused by styrene, metabolism of styrene may be enhanced through the ALDH2 genes, and therefore genetic toxicity caused by styrene is reduced. As a result, targeting the metabolic detoxication function of the ALDH2 genes, the ALDH2 genes can be used for preparing healthcare products or medicine for preventing genetic toxicity caused by styrene.

Description

technical field [0001] The invention belongs to the field of gene function and application, and relates to the application of ALDH2 gene in the preparation of drugs for preventing genotoxicity caused by styrene. Background technique [0002] Styrene is an important industrial raw material used in the production of plastics, fiberglass, pipes, automotive hull parts and food packaging boxes. Not only professional workers in production workshops may be exposed to high concentrations of styrene, ordinary people may also be exposed to styrene through smoking, car exhaust, carpets, and food packaging boxes. Therefore, whether styrene has adverse effects on human health, especially its carcinogenicity has received widespread attention. [0003] Previous studies have shown that styrene has clear carcinogenicity in mouse experiments, but the results of epidemiological investigations in the population are not consistent. In the study of the genotoxicity of styrene, it was further fo...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61P39/02A23L33/10
Inventor 翁祖铨万梅郑丽婷严芬
Owner FUZHOU UNIV
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