Preparation method of lactobacillus special for milk

A technology for lactic acid bacteria and dairy cows, which is applied in the field of preparation of lactic acid bacteria for dairy cows. It can solve the problems of ineffective biological activity, poor reproductive activity, and long-term settlement of lactic acid bacteria. Active effect

Inactive Publication Date: 2016-01-20
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Lactic acid bacteria are the dominant probiotics in the gastrointestinal tract of ruminants. They have a variety of biological activities and functions. The lactic acid bacteria contained are mostly derived from soil, water sources, plants and dairy products. The lactic acid bacteria cannot settle in the gastrointestinal tract of animals for a long time, have poor reproductive activity, and are inhibited by antibiotics, so they cannot effectively exert their biological activity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: a kind of preparation method of milk-cow special lactic acid bacteria, is characterized in that mainly comprises the following steps:

[0020] a. Extraction of rumen lactic acid bacteria:

[0021] Using lactic acid bacteria to select SL medium to isolate and identify the flora from the rumen juice of healthy cows, and isolate the lactic acid bacteria strains;

[0022] b. Identification of strain environmental stress resistance:

[0023] The strains isolated in step a were first identified by the API50CHL biochemical identification kit, and 30 strains were selected according to their growth characteristics, and were cultured at pH2, 0.2% bile salts, and 25 ℃ - 40 ℃ environment, and screened out. 3 strains of Lactobacillus and Lactobacillus fermentum with good growth and strong environmental stress resistance;

[0024] c. Antibiotic resistance and bacteriostatic test of isolates:

[0025] Drug resistance test: The selected strains with good environmental ...

Embodiment 2

[0030] Embodiment 2: a kind of preparation method of milk-cow special lactic acid bacteria, is characterized in that mainly comprises the following steps:

[0031] a. Extraction of rumen lactic acid bacteria:

[0032] Using lactic acid bacteria to select SL medium to isolate and identify the flora from the rumen juice of healthy cows, and isolate the lactic acid bacteria strains;

[0033] b. Identification of strain environmental stress resistance:

[0034] The strains isolated in step a were first identified by the API50CHL biochemical identification kit, and 30 strains were selected according to their growth characteristics, and were cultured at pH 2, 0.3% bile salts, and 25 ℃ - 40 ℃ environment, and screened out. 4 strains of Lactobacillus and Lactobacillus fermentum with good growth and strong environmental stress resistance;

[0035] c. Antibiotic resistance and bacteriostatic test of isolates:

[0036] Drug resistance test: The selected strains with good environmental...

Embodiment 3

[0041] Embodiment 3: a kind of preparation method of milk-cow special lactic acid bacteria, it is characterized in that mainly comprising the following steps:

[0042] a. Extraction of rumen lactic acid bacteria:

[0043] Using lactic acid bacteria to select SL medium to isolate and identify the flora from the rumen juice of healthy cows, and isolate the lactic acid bacteria strains;

[0044] b. Identification of strain environmental stress resistance:

[0045] The strains isolated in step a were first identified by the API50CHL biochemical identification kit, and 30 strains were selected according to their growth characteristics, and were cultured at pH 3, 0.4% bile salts, and 25 ℃ - 40 ℃ environment, and screened out. 2 strains of Lactobacillus and Lactobacillus fermentum with good growth and strong environmental stress resistance;

[0046] c. Antibiotic resistance and bacteriostatic test of isolates:

[0047] Drug resistance test: The selected strains with good environme...

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Abstract

The invention discloses a preparation method of a lactobacillus special for milk. The preparation method mainly comprises the following steps of extracting rumen lactobacillus, identifying strain environment resistance, testing isolated strain drug resistance and bactriostasis, performing enlarged culture of lactobacillus and preparing active microbial agent. Compared with the prior art, the lactobacillus has the advantages that the lactobacillus can be settled down in the gastrointestinal tract of an animal for a long time, the breeding activity is strong, the inhibition effect of antibiotics is not obvious, and the bioactive action can effectively come into play.

Description

technical field [0001] The invention belongs to the field of lactic acid bacteria preparation, and in particular relates to a preparation method of lactic acid bacteria special for dairy cows. Background technique [0002] The gastrointestinal tract of ruminants is rich in microbial flora, and lactic acid bacteria are the main inherent dominant flora, which play an important physiological role in the digestion and absorption of nutrients and resistance to intestinal infections. A large number of studies have shown that the lactic acid produced by lactic acid bacteria can reduce intestinal pH, acidify the intestinal tract, improve the utilization rate of calcium, phosphorus and iron, and maintain the absorption rate of vitamin D. Broad inhibitory effect; Lactobacillus metabolites also activate macrophages, NK cells and B cells. Improve the body's anti-infection immune function. Therefore, lactic acid bacteria, as the dominant bacteria in the gastrointestinal tract, are often...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/04
Inventor 蒋建军剡根强王鹏雁杨龙龙剡文莉
Owner SHIHEZI UNIVERSITY
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