Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for improving production efficiency of glutamic acid fermentation by changing inoculation mode

A technology of production efficiency and glutamic acid, applied in the field of microbial fermentation, can solve the problems of prolonging the fermentation period, increasing the contamination, and long seed cultivation time, and achieving the effects of improving production efficiency, reducing the number of dockings, and shortening the fermentation period

Inactive Publication Date: 2016-01-20
LIANG SHAN LING HUA BIOTECH CO LTD
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the seed bottle is connected to the primary culture tank, the seed culture time is too long, which greatly prolongs the entire fermentation cycle.
At the same time, since the seed bottle is exposed to the air when it is connected to the seed tank, and it is manually operated, the possibility of bacterial contamination is greatly increased

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving production efficiency of glutamic acid fermentation by changing inoculation mode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The present invention improves the method for glutamic acid fermentation production efficiency by changing inoculation mode, comprises the following steps:

[0017] 1) A 1.5L fermentative glutamic acid seed bottle was inserted into a 5t zero-grade seed tank, cultivated until the OD value was 0.55, and the control culture time was 32 hours;

[0018] 2) Connect the zero-level seed tank of step 1) to a 5t first-level seed tank, and each zero-level seed tank is connected to two first-level seed tanks, cultivate to an OD value of 0.6, and control the cultivation time for 12 hours;

[0019] 3) Connect the first-level seed tank of step 2) to a 5t second-level seed tank, and each first-level seed tank is connected to a second-level seed tank, cultivated to an OD value of 0.9, and the control culture time is 12h;

[0020] 4) All the secondary seed tanks in step 3) are connected to the fermenter, and the fermentation time is controlled to be 28 hours to complete the fermentation ...

Embodiment 2

[0023] 1) 3L fermentative glutamicum seed bottles were inserted into 10t zero-grade seed tanks, cultivated to an OD value of 0.8, and the control culture time was 33 hours;

[0024] 2) Connect the zero-level seed tank of step 1) to a 10t first-level seed tank, and each zero-level seed tank is connected to four first-level seed tanks, cultivated to an OD value of 0.7, and the control culture time is 13h;

[0025] 3) Connect the first-level seed tank of step 2) to a 10t second-level seed tank, each first-level seed tank is connected to a second-level seed tank, and cultivate until the OD value is 1.0, and the control culture time is 13h;

[0026] 4) All the secondary seed tanks in step 3) are connected to the fermenter, and the fermentation time is controlled to be 30 hours to complete the fermentation cycle.

[0027] From step 2) to step 4) as a fermentation cycle, the whole fermentation cycle is 56h.

Embodiment 3

[0029] 1) 3L fermentative glutamicum seed bottles were inserted into 10t zero-grade seed tanks, cultivated until the OD value was 1.0, and the control culture time was 34 hours;

[0030] 2) Connect the zero-level seed tank of step 1) to a 10t first-level seed tank, and each zero-level seed tank is connected to 6 first-level seed tanks, cultivate to an OD value of 0.7, and control the cultivation time for 14 hours;

[0031] 3) Connect the first-level seed tank of step 2) to a 10t second-level seed tank, each first-level seed tank is connected to a second-level seed tank, cultivate to an OD value of 1.2, and control the cultivation time to 14h;

[0032] 4) All the secondary seed tanks in step 3) are connected to the fermenter, and the fermentation time is controlled to be 29 hours to complete the fermentation cycle.

[0033] From step 2) to step 4) as a fermentation cycle, the whole fermentation cycle is 57h.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for improving the production efficiency of glutamic acid fermentation by changing an inoculation mode and belongs to the technical field of the microorganism fermentation. The method comprises the following steps of: inoculating seeds in a seed bottle into zero-level seed tanks, connecting the zero-level seed tanks into first-level seed tanks, connecting 2-10 first-level seed tanks into each zero-level seed tank, connecting second-level seed tanks into the first-level seed tanks, connecting one second-level seed tank into each first-level seed tank, connecting all the second-level seed tanks into a fermentation tank, controlling the fermentation time to be 28-30 hours and finishing the fermentation period; the seeds in the seed bottle is glutamic acid fermentation bacillus. The method disclosed by the invention has the advantages that by addition of the zero-level seed tanks in the front of the first-level seed tanks, the butting times of the seed bottle and the fermentation tanks are reduced, further the probability of contamination is further reduced, the amount of seeds inoculated to the first-level seed tanks after being cultured by the zero-level seed tank is increased, the culture period of the first-level seed tanks is shortened, the fermentation period is shortened and the production efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for improving the production efficiency of glutamic acid fermentation by changing the inoculation method. Background technique [0002] In glutamic acid fermentation production, since fermentation requires a large amount of seeds, a certain amount of seeds are always cultivated in seed tanks before being inoculated into fermenters for fermentation. However, the seed culture time is too long after the seed bottle is connected to the primary culture tank, which greatly prolongs the entire fermentation period. Simultaneously, since the seed bottle is exposed to the air when it is connected to the seed tank, and it is manually operated, the possibility of bacterial contamination is greatly increased. Contents of the invention [0003] The purpose of the present invention is to provide a method for improving the production efficiency of glutamic ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/14
Inventor 杨玉岭岳希金满德恩郭脉海张爱敏殷慧慧
Owner LIANG SHAN LING HUA BIOTECH CO LTD