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Method for producing Chlamydosporium crassicarpa granule by solid state fermentation of silkworm excrement

A solid-state fermentation and Verticillium spore technology, applied in the biological field, can solve the problems of unstable control effect, low bacterial cell survival rate, and great influence of inoculants, and achieves the advantages of eliminating the granulation process, high survival rate and low cost. Effect

Active Publication Date: 2019-01-25
李艳华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the problems that the existing Puccinia chlamydobacteria have few dosage forms, the preparation price is high, the external environment has a relatively large impact on the bacteria agent, the survival rate of the bacteria is low after being applied to the soil, it is difficult to reproduce, and the control effect is unstable.

Method used

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  • Method for producing Chlamydosporium crassicarpa granule by solid state fermentation of silkworm excrement

Examples

Experimental program
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Effect test

Embodiment 1

[0031] The method for producing Verticillium chlamydospora granules by solid-state fermentation of silkworm excrement of the present embodiment comprises the following steps:

[0032] a. Bacteria activation:

[0033] Prepare PDA slant medium and inoculate Pochonia chamydosporia=Verticillium chlamydosporium CGMCC 14131, cultivate at room temperature for 5 days, then wash the conidia of Trichoderma harzianum on the PDA slant medium with sterile water, and prepare a concentration of for 10 8 spores / mL of spore suspension;

[0034]Weigh 10 parts of silkworm excrement and 10 parts of water in parts by weight, mix well and balance the water for 60 minutes, stir well and put it into a 500mL Erlenmeyer flask, each bottle is filled with 250g, sealed with a breathable film, sterilized at 121°C for 120min, and cooled After reaching room temperature, inoculate 10 mL / bottle of Verticillium chlamydospore spore suspension, and culture at 26°C until the mycelium is covered with silkworm exc...

Embodiment 2

[0041] The method for producing Verticillium chlamydospora granules by solid-state fermentation of silkworm excrement of the present embodiment comprises the following steps:

[0042] a. Bacteria activation:

[0043] Prepare PDA slant medium and inoculate Pochonia chamydosporia=Verticillium chlamydosporium CGMCC 14131, cultivate at room temperature for 5 days, then wash the conidia of Trichoderma harzianum on the PDA slant medium with sterile water, and prepare a concentration of for 10 8 spores / mL of spore suspension;

[0044] Weigh 10 parts of silkworm excrement and 6 parts of water in parts by weight, mix well and balance the water for 60 minutes, stir well and put it into a 500mL Erlenmeyer flask with 250g of material in each bottle, seal it with a gas-permeable film, sterilize at 121°C for 120min, cool After reaching room temperature, inoculate 20 mL / bottle of Verticillium chlamydospore spore suspension, and culture at 26°C until the mycelium is covered with silkworm ex...

Embodiment 3-10

[0051] The different implementations of Examples 3-10 are shown in Table 1, and the rest of the operations are the same as in Example 1.

[0052] After implementation according to different examples, sampling is carried out according to the method of 5.3.3 "solid preparation sampling" in GB / T 1605-2001, and the drying loss is determined according to the pesticide drying loss method specified in GB / T 30361-2003. The number of chlamydospores, the number of conidia and the rate of viable spores (%) of Verticillium chlamydospore granules were determined. The detection method of Chlamydosporum is one-by-one counting method under the microscope, conidia are counted by hemocytometer plate counting method, and the viable spore rate is by the spore germination rate method on 0.5% malt extract medium. The results are shown in Table 1. After testing, the chlamydospores in the final product can reach 9.8×10 6 individual / g, conidia up to 9.9×10 9 pcs / g. From the detailed description of ...

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Abstract

The invention provides a method for producing Chlamydosporium crassicarpa granule by solid state fermentation of silkworm excrement, which comprises the steps of bacterial species activation, liquid species preparation, solid state fermentation, solid state fermentation product drying and the like. A liquid strain is prepared by activating that strain, and the culture medium component in the solidfermentation step is silkworm excrement and water. The invention adopts solid state fermentation of silkworm excrement to produce Chlamydosporium chlamydosporum granule, which not only makes good useof silkworm excrement and effectively implements recycling and reuse of resources, but also produces Chlamydosporum chlamydosporum granule with minimum cost and simplified process.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for producing Verticillium chlamydospora granules by solid-state fermentation of silkworm excrement. Background technique [0002] Plant parasitic nematodes are one of the important plant pathogens, which generally occur in the world, have a wide range of hosts, and cause serious damage. In addition, nematodes are also one of the important causes of plant fungal and bacterial diseases. Among them, root-knot nematode Meloidogyne spp. and cyst nematode Heterodera spp. are the most serious plant parasitic nematodes. Because of their wide distribution and multiple hosts, they have become important pathogens that threaten agricultural production. At present, the control of plant parasitic nematodes is still dominated by chemical agents, but they are highly toxic, pollute the environment, and pose a potential threat to human and animal health. As more and more...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02C12R1/645
CPCC12N1/14C12P1/02C12R2001/645C12N1/145
Inventor 李文鹏陈芬玲朱芳乐朱云九
Owner 李艳华
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