Alcohol and succinic acid production method by fermenting cellulosic raw material
A technology of cellulose and succinic acid, which is applied in the field of producing ethanol and succinic acid by fermentation of cellulose raw materials, can solve the problems of being unused, and achieve low production costs, reduced emissions, and wide sources of raw materials Effect
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Embodiment 1
[0023] Example 1 Preparation of sugar solution with corn stalks as raw material
[0024] Using corn stalks as raw materials to prepare sugar liquid comprises the following steps:
[0025] (1) Take 100 kg of corn stalks, remove the leaves and roots by hand, and process them with a straw feed kneading machine and a chip grinder. The aperture of the bottom discharge screen is Φ5, and the corn stalk powdery material is obtained.
[0026] (2) Using a cylindrical reactor with a volume of 0.8 cubic meters and a rotation speed of 1 rpm, 100 kilograms of crushed corn stalks were added to it, and 600 kilograms of dilute alkali solution with a concentration of 1.8% sodium hydroxide was added. Heating to 120°C, keeping the temperature for 1 hour, cooling down, and releasing the material.
[0027] (3) The material after the alkali treatment is squeezed dry with a screw squeezer, and the solid phase is washed with hot water until the pH value of the material is less than 9.
[0028] (4) J...
Embodiment 2
[0031] The establishment of embodiment 2 ethanol fermentation system
[0032] The establishment of ethanol fermentation system comprises the following steps:
[0033] (1) Plate culture: the plate medium consists of 3% glucose, 0.5% yeast extract, 0.5% peptone, and 2% agar. Alcoholic yeast strains were inoculated into the medium, and cultured at 33°C for 28 hours.
[0034] (2) Shake flask seed culture: the composition of the shake flask seed medium is glucose 3%, yeast extract 0.5%, peptone 0.15%, corn steep liquor 0.5%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.06%, and tap water preparation. Inoculate the alcoholic yeast strain cultured on the plate into the medium, and culture at 33° C. for 32 hours, with the rotating speed of the shaker at 200 rpm.
[0035] (3) seed tank seed culture: medium composition is the concentrated sugar solution that embodiment 1 obtains, and add-on is to make the total sugar content in medium be 5%, yeast extract 0.2%, peptone 0....
Embodiment 3
[0038] Example 3 Succinic acid fermentation system and its coupling with ethanol fermentation system
[0039] The production method of succinic acid comprises the following steps:
[0040] (1) Plate culture: the succinic acid bacteria species Actinobacillus succinicum was inoculated into tryptone soybean agar medium, and cultured anaerobically at 35° C. for 28 hours.
[0041] (2) shake flask seed culture: medium composition is the concentrated sugar solution that embodiment 1 obtains, and add-on is to make the total sugar content in medium be 3%, yeast extract 0.4%, peptone 0.2%, corn steep liquor 0.3%, Na 2 CO 3 0.9%, NaH 2 PO 4 0.7%, K 2 HPO 4 1.0%, Na 2 HPO 4 0.2%, NaCl0.2%, tap water preparation. Inoculate the succinic acid bacteria cultured on the plate into a serum bottle containing 200ml of the above-mentioned medium for shaking flask culture, cultivate at 35°C for 15 hours, and the shaker speed is 180rpm.
[0042] (3) Seed tank culture: the medium composition i...
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