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A method for the determination of paralytic shellfish toxin in aquatic products

A shellfish toxin and paralysis technology, applied in the field of aquatic product detection, can solve the problem of large differences in toxin recovery rate, achieve the effect of satisfying sensitivity and precision, saving time and cost, and reducing consumption

Inactive Publication Date: 2017-03-22
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the LC-MS detection method for PSTs in the world still has certain deficiencies, such as the recovery rate of each component of the toxin in the pretreatment method is very different, and there is no suitable purification method to well limit the matrix effect on the determination of components. influence etc.

Method used

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  • A method for the determination of paralytic shellfish toxin in aquatic products
  • A method for the determination of paralytic shellfish toxin in aquatic products
  • A method for the determination of paralytic shellfish toxin in aquatic products

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Embodiment 1

[0032] 1. Materials and methods

[0033] 1.1 Main instruments and reagents

[0034]Liquid chromatography tandem mass spectrometer: Thermo TSQ Quantum Ultra triple quadrupole mass spectrometer, electrospray ionization source (ESI) (Thermo Fisher Scientific, USA);

[0035] Liquid chromatography column: TSK-gel Amide-80 hydrophilic column (150×2mm, 3μm):

[0036] Tissue masher: IKA T-18 (IKA, Germany);

[0037] Vortex instrument: Haimen Qilin Bell Company;

[0038] Ultrasonic oscillator: Ningbo Xinzhi Company;

[0039] Refrigerated centrifuge: Heraeus Multifuge 1L-R (Thermo Fisher Scientific, Germany);

[0040] Freeze dryer: Heto Power Dry LL3000, Shanghai Huifen Electronic Technology Co., Ltd.;

[0041] Nitrogen blowing instrument: HGC-24 Xi'an Jingda Testing Equipment Co., Ltd.;

[0042] Ultrasonic cleaner: Shanghai Kedao Ultrasonic Instrument Co., Ltd.;

[0043] Ultrapure water machine: Milli-Q, Millipore, Germany;

[0044] 1.2 Standards and reagents

[0045] Eight ki...

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PUM

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Abstract

The invention provides a method for measuring paralysis shellfish poison in aquatic products. The method comprises the steps of extraction, freezing, redissolving, purification and liquid chromatography-tandem mass spectrometer detection. The method has the advantages that sample pretreatment is simple, quick and economical, and sensitivity and recovery rate are high. The method is suitable for simultaneous, qualitative and quantitative detection of multiple kinds of paralysis shellfish poison in aquatic products, and provides effective technical support for guaranteeing quality safety of shellfish aquatic products in China.

Description

technical field [0001] The invention relates to the field of aquatic product detection, in particular to a method for determining paralytic shellfish toxin in aquatic products. Background technique [0002] Paralytic shellfish toxins (PSTs) are a class of highly water-soluble small molecule secondary metabolites produced by planktonic algae or microorganisms in water bodies. Its basic structure is two guanidine-based four-hydrated purine stacked six-membered rings. According to the different substituent groups, it is mainly divided into four categories: ①Carbamate toxins, including saxitoxin (STX), neoSTX (NEO) and genital toxins 1-4 (Gonyautoxins, GTXl-4); ②N-sulfonyl carbamoyl toxins (N-sulfocarbamoyl toxins), including GTX5 (B1), GTX6 (B2) and C1-C4; ③ decarbamoyl toxins ( Decarbamoyl toxins), including dcSTX, dcneoSTX, dcGTX1-4; ④ Deoxydecarb-amoyl toxins (Deoxydecarb-amoyl toxins), including doSTX, doneoSTX and doGTX1, among which carbamate toxins are a more important...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 杨宪立周磊聂冬霞赵志勇武爱波
Owner SHANGHAI ACAD OF AGRI SCI
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