A kit for detecting cancer marker microRNA

Abstract

The invention relates to a nanoprobe capable of analysis and detection of activity of cancer marker MicroRNAs and MicroRNAs in cancer cells and a preparation method therefor. The nanoprobe is a novel four-helical DNA molecule probe. The nanoprobe contains three functional parts: a target object identification area loaded with special design, a trigger area triggering a cyclic amplification reaction and a template area for a polymerization-shearing amplification reaction. The nanoprobe is introduced into a surface enhanced Raman scattering (SERS) analysis detection technology, a four-helical molecule probe-SERS detection method is developed, and high-sensitivity detection of microRNAs can be achieved. Combined with the aptamer identification technology, the four-helical molecule probe-SERS detection method has good specificity, the sample treatment process is simplified, the selectivity is good, the accuracy is high, and the method is an ideal method for detecting the tumor cell marker microRNAs and microRNAs in cancer cells. The detection method and kit can distinguish cancer cells and normal cells, and has advantages of simple operation, and high-sensitivity and high-specificity detection of the cancer marker microRNAs, and thus the kit has good application prospects.

Description

technical field [0001] The present invention relates to deoxyribonucleic acid (abbreviated as DNA) cyclic amplification technology, specifically, utilizes the cyclic amplification technology of four-helix structure DNA to carry out surface-enhanced Raman scattering on cancer marker MicroRNA (hereinafter referred to as MicroRNA) and MicroRNA in cancer cells (SERS) identification and immunodetection kits and testing methods. Background technique [0002] MicroRNA is a class of minimal functional coding RNA ubiquitous in animals and plants, endogenous single-stranded small molecules with a length of about 20-24 nucleotides, which have a variety of important regulatory functions in cells. It is of great significance for in-depth exploration of disease pathogenesis, disease diagnosis, and development of gene therapy drugs. MicroRNA research has been rated as one of the top ten technologies of the year and the major breakthrough of the year by Science magazine. MicroRNA regulate...

AI Technical Summary

Problems solved by technology

[0003] The concentration of tumor marker MicroRNA in the serum of patients with early cancer is only 10 -11 M-10 -15 M, the lowest concentration that current clinical immunoassays can detect is only 10 -12 M, unable to meet the requirements of early diagnosis of modern medicine

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