Method for preparing homogenous liquid particle strain by utilizing nicandra seeds

A technology of pseudophysalis and liquid particles, which is applied in the field of preparation of liquid strains, and can solve the problem of unsatisfactory physical properties such as density, size, and uniformity of bacterial balls, long cultivation cycles of solid strains, and uneven product quality. problem, to achieve the effects of shortening the production cycle, fast germination, and short seed production time

Active Publication Date: 2016-02-17
KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solid strains have a long cultivation cycle, poor stress resistance, high pollution rate, and uneven product quality
Although conventional liquid strains solve some problems of solid strains, can significantly shorten the cultivation cycle, enhance the vitality and stress resistance of the strains, reduce the contamination rate of the strains, and improve the uniformity of the strains, there are still deficiencies. For example, the density, size, and uniformity of the bacterial balls in the liquid strains obtained by fermentation are not very ideal. If the bacterial balls are broken by stirring and shearing, the uniformity of the obtained strains will be more ideal. However, high-speed The shear force of mixing, crushing and beating is extremely harmful to the strains, and has a serious impact on the overall quality of the strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Wrap 1.0 g of pseudophysalis with kraft paper, place in a high-pressure steam sterilizer at 120°C, and sterilize for 25 minutes for later use. Prepare a PDA slant medium, the composition of which is calculated per 1000ml of water: 200g of potatoes, 20g of glucose, 13g of agar powder, and a pH value of 7.0. After preparation, the culture medium was placed in a high-pressure steam sterilizer at 120° C., and sterilized for 25 minutes for use. Pour the sterilized pseudophysalis into the sterilized PDA slant medium, spread evenly to form the seed layer, and then inoculate the target test tube bacteria on the surface of the seed layer, the inoculation area is 0.5×0.5cm 2 , and cultivated in the dark at 23°C for 7 days, until the target mycelium is covered with the surface of the pseudophysalis seeds. Then, prepare a semi-flowing liquid medium, the composition of which is calculated per 1000ml: 200g of potatoes, 20g of glucose, KH 2 PO 4 0.5g, K 2 HPO 4 0.5g, MgSO 4 0.5g...

Embodiment 2

[0032] Wrap 1.1 g of pseudophysalis with kraft paper, place in a high-pressure steam sterilizer at 125°C, and sterilize for 20 minutes for later use. Prepare PDA slant medium, the composition of which is calculated per 1000ml of water: 220g of potatoes, 18g of glucose, 12g of agar powder, and pH 7.5. After preparation, the medium was placed in a high-pressure steam sterilizer at 125° C., and sterilized for 20 minutes for later use. Pour the sterilized pseudophysalis into the sterilized PDA slant medium, spread it evenly to form the seed layer, and then inoculate the target test tube bacteria on the surface of the seed layer, the inoculation area is 0.6×0.6cm 2 , and cultivated in the dark at 25°C for 6 days, until the target hyphae are covered with the surface of the pseudophysalis seeds. Then, prepare a semi-flowing liquid medium, the composition of which is calculated per 1000ml: potatoes 220g, glucose 18g, KH 2 PO 4 0.6g, K 2 HPO 4 0.4g, MgSO 4 0.4g, 3.8g of agar powd...

Embodiment 3

[0034] Wrap 0.9 g of pseudophysalis with kraft paper, place in a high-pressure steam sterilizer at 122°C, and sterilize for 21 minutes for later use. Prepare a PDA slant medium, the composition of which is calculated per 1000ml of water: 180g of potatoes, 22g of glucose, 15g of agar powder, and a pH value of 6.5. After preparation, the culture medium was placed in a high-pressure steam sterilizer at 122° C., and sterilized for 21 minutes for later use. Pour the sterilized pseudophysalis into the sterilized PDA slant medium, spread it evenly to form the seed layer, and then inoculate the target test tube bacteria on the surface of the seed layer, the inoculation area is 0.4×0.4cm 2, and cultivated in the dark at 22°C for 6 days, until the target mycelium is covered with the surface of the pseudophysalis seeds. Then, prepare a semi-flowing liquid medium, the composition of which is calculated per 1000ml: potatoes 180g, glucose 22g, KH 2 PO 4 0.4g, K 2 HPO 4 0.6g, MgSO 4 0....

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Abstract

The invention discloses a method for preparing a homogenous liquid particle strains by utilizing nicandra seeds. The method comprises the steps of seed sterilization, slope culture medium preparation, inoculation, liquid medium preparation and shaking cultivation; specifically, packaging the nicandra seeds and sterilizing; preparing a PDA slope culture medium and sterilizing; putting the nicandra seeds into the slope culture medium, uniformly paving the nicandra seeds to be as a seed layer; inoculating targeted test tube strains onto the surface of the seed layer, and cultivating the strains at 22-25 DEG C in dark for 6-8 days till targeted hypha coats the surfaces of the nicandra seeds; preparing the liquid medium by per 1000ml from the following ingredients: 180-220 g of potato, 18-22 g of glucose, 0.4-0.6 g of KH2PO4, 0.4-0.6 g of K2HPO4, 0.4-0.6 g of MgSO3 and 3.2-3.8 g of agar powder, wherein a PH value of the liquid medium is 6.5-7.5; inoculating the nicandra seeds coated with the targeted hypha into the liquid medium, and performing shaking cultivation on the nicandra seeds at 22-25 DEG C in dark for 8-12 days, thereby obtaining targeted homogenous liquid particle strains. The strains prepared by the method provided by the invention have the advantages that the distribution of ball strains is uniform, and the homogenous liquid strains can be obtained without external-force crushing and pulping; the damage of external force on the ball strains is reduced, and the integral quality of the ball strains is improved.

Description

technical field [0001] The invention belongs to the technical field of preparation of liquid strains, and in particular relates to a method for preparing homogeneous liquid granular strains from pseudophysalis. Background technique [0002] The strains currently used for industrial cultivation of edible fungi include solid strains and liquid strains. Solid strains have a long cultivation period, relatively poor stress resistance, high pollution rate, and uneven product quality. Although conventional liquid strains solve some problems of solid strains, can significantly shorten the cultivation cycle, enhance the vitality and stress resistance of the strains, reduce the contamination rate of the strains, and improve the uniformity of the strains, there are still deficiencies. For example, the density, size, and uniformity of the bacterial balls in the liquid strains obtained by fermentation are not very ideal. If the bacterial balls are broken by stirring and shearing, the un...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 李建英华蓉郭永红刘绍雄罗孝坤尚陆娥
Owner KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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