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Method for identifying odontobulis mpotamophila family

A technology of sandfish and rivers, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., to reduce errors, avoid damage to fish bodies, and ensure accuracy

Inactive Publication Date: 2016-02-17
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the use of microsatellite marker technology for family identification and paternity identification has been applied in aquatic organisms such as prawn (Fenneropenaeuschinensis), rainbow trout (Oncorhynchusmykiss), turbot (Scophthalmus maximus), and the family identification and kinship of the river sand pond snakehead at home and abroad. Relationship identification has not been reported yet

Method used

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  • Method for identifying odontobulis mpotamophila family
  • Method for identifying odontobulis mpotamophila family
  • Method for identifying odontobulis mpotamophila family

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The 7 families to be tested are numbered as A, B, C, D, E, F, G, the parents and 10 offspring individuals of family D are randomly selected, and 2 individuals of river channa from unknown sources are selected, and recorded For unknown 1 and unknown 2, the genotype bp number of the individual to be tested is obtained according to the above method, and appropriate manual correction is performed to establish a family genotype information table. Table 4 shows the genotype of 12 pairs of primers among family D individuals data sheet.

[0048] Table 412 genotype data table for primers

[0049]

[0050]

[0051] Note: The number represents the size of the gene fragment

[0052] Convert the genotype data table into the POPGENE format required by the POPGENE1.32 software, use the POPGENE1.32 software to analyze the genetic distance of the individual to be tested, and obtain the genetic consistency, use EXCEL to transpose the genetic consistency table and convert it into ...

Embodiment 2

[0054] Randomly select the parents of 7 families and the offspring of the separated families, obtain the gene bp numbers of the parents and the offspring of each family according to the above method, and perform appropriate manual corrections to establish a family genotype information table, Table 5 It is the genotype data table of 12 pairs of primers among some individuals.

[0055] Table 512 genotype data table for primers

[0056]

[0057]

[0058] Note: The number represents the size of the gene fragment

[0059] According to the method shown in Example 1, cluster analysis was carried out on the parents and offspring of 7 families, and a cluster diagram was obtained. figure 2 It is the clustering situation of 7 families. It can be seen from the clustering diagram that the parents and offspring groups of each family are correctly gathered together, which is completely consistent with the known situation. Therefore, the selected microsatellite markers can distinguis...

Embodiment 3

[0061] According to the method shown in Example 1, the genotype data of the parents of 7 families and 100 mixed offspring were obtained, and the genotype database was established, and the parents and offspring of the seven families were aggregated with POPGENE1.32, EXCEL and NTSYS software. class analysis, image 3 For the clustering case. It can be seen from the figure that all 100 mixed offspring can be assigned to the family of a certain parent, so it can be determined that all 100 mixed offspring individuals can find their corresponding parents.

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Abstract

The invention relates to the technical field of fish molecular marker and genetic resource identification, in particular to a method for identifying an odontobulis mpotamophila family. The method comprises the steps of 1, building and breeding an odontobulis mpotamophila family to be tested; 2, extracting DNAs of parents and hybrids of the odontobulis mpotamophila family to be tested; 3, screening and synthetizing polymorphism microsatellite primers of odontobulis mpotamophila; 4, amplifying microsatellite primers of the parents and hybrids of odontobulis mpotamophila; 5, identifying systems and genetic relationships of the odontobulis mpotamophila. According to the method, different systems can be accurately and rapidly identified, paternity testing can be performed on the odontobulis mpotamophila, artificial breeding is optimized, in-breeding degeneration is avoided, technical support is provided for building the family tree of odontobulis mpotamophila cultured stocks and formulating scientific population genetic management strategy, and the reliable method is provided for later large-scale family identification and paternity testing of odontobulis mpotamophila.

Description

technical field [0001] The invention relates to the technical field of fish molecular markers and identification of germplasm resources, in particular to a method for identifying the family of the river sand pond snakehead. Background technique [0002] Odontobutis potamophila belongs to the order Perciformes, family Odontobutidae, Odontobutis genus, commonly known as Pu fish, Tubu fish, tiger shark, tiger head nerd. In my country, it is widely distributed in the middle and lower reaches of the Yangtze River, Qiantang River, Minjiang River and other water systems, and belongs to China's endemic species. The fish is a small carnivorous fish that dwells in freshwater bottom. The meat is delicate and tender. At present, the polyculture and interculture of S. chinensis have been carried out sporadically across the country. However, in the process of producing the fish fry, the production unit does not pay attention to the management of the germplasm resources of the fish, which...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 尹绍武王佩佩丁严冬于兴达贾秀琪
Owner NANJING NORMAL UNIVERSITY
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