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Tissue culture rapid propagation medium and tissue culture rapid propagation method of Mingyue grass

A tissue culture and culture medium technology, applied in the field of plant tissue culture, to achieve the effects of solving seasonal restrictions, shortening the reproduction cycle and high quality seedlings

Active Publication Date: 2017-09-22
钦州市林业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the domestic research on the breeding of Mingyuecao by tissue culture technology

Method used

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  • Tissue culture rapid propagation medium and tissue culture rapid propagation method of Mingyue grass
  • Tissue culture rapid propagation medium and tissue culture rapid propagation method of Mingyue grass
  • Tissue culture rapid propagation medium and tissue culture rapid propagation method of Mingyue grass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Bright moon grass tissue culture rapid propagation medium, including axillary bud induction medium, subculture medium, strong seedling medium and rooting medium, the preparation method is:

[0041] Axillary bud induction medium is MS+6-BA0.8mg / L+NAA0.1mg / L+sucrose 30g / L+agar powder 3-4g / L, pH5.5, 1.1kg / cm 2 Autoclave for 20 minutes;

[0042] The subculture medium is MS+6-BA0.5mg / L+NAA0.05mg / L+AD0.2mg / L+sucrose 30g / L+agar powder 3-4g / L, pH5.8, through 1.1kg / cm 2 Autoclave for 20 minutes;

[0043] Strong seedling medium is MS+6-BA0.2mg / L+NAA0.02mg / L+sucrose 30g / L+agar powder 3-4g / L, pH5.8, 1.1kg / cm 2 Autoclave for 20 minutes;

[0044] Rooting medium is 1 / 2MS 0 + sucrose 30g / L+ agar powder 3-4g / L, pH5.8, after 1.1kg / cm 2 Autoclave for 20 minutes.

Embodiment 2

[0046] Bright moon grass tissue culture rapid propagation medium, including axillary bud induction medium, subculture medium, strong seedling medium and rooting medium, the preparation method is:

[0047] Axillary bud induction medium is MS+6-BA0.8mg / L+NAA0.1mg / L+sucrose 30g / L+agar powder 3-4g / L, pH5.8, 1.1kg / cm 2 Autoclave for 15 minutes;

[0048] The subculture medium is MS+6-BA0.5mg / L+NAA0.05mg / L+AD0.2mg / L+sucrose 30g / L+agar powder 3-4g / L, pH5.5, through 1.1kg / cm 2 Autoclave for 15 minutes;

[0049] Strong seedling medium is MS+6-BA0.2mg / L+NAA0.02mg / L+sucrose 30g / L+agar powder 3-4g / L, pH5.5, through 1.1kg / cm 2 Autoclave for 15 minutes;

[0050] Rooting medium is 1 / 2MS 0 + sucrose 30g / L+ agar powder 3-4g / L, pH5.5, after 1.1kg / cm 2 Autoclave for 15 minutes.

Embodiment 3

[0052] Tissue culture rapid propagation method of Mingyue grass:

[0053] (1) selection of explants: in spring, the new shoots with latent buds on the mother plant of Mingyue Grass are explants;

[0054] (2) Disinfection of explants: cut off the branches and leaves, rinse with tap water for 2-3 minutes, then wash with detergent for 1-2 times, then rinse with tap water for 5 minutes, and then rinse with 0.1% HgCl 2 The solution was disinfected for 10 minutes, and finally rinsed with sterile water 5 times, drained and set aside;

[0055] (3) Axillary bud induction culture: remove the stem tip from the sterilized branches, cut the remaining branches into stem segments with 1-2 axillary buds in each section, inoculate them on the induction medium and put them into the culture room for aseptic culture. The base is MS+6-BA0.8mg / L+NAA0.1mg / L+sucrose 30g / L+agar powder 3g / L, pH5.6, MS medium is 1.1kg / cm 2 Autoclave for 20 minutes, culture conditions are temperature 25-30°C, light in...

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Abstract

The invention discloses a tissue-culture rapid propagation medium and a tissue-culture rapid propagation method of A. sciatica. The method comprises explant disinfection, axillary bud induction culture, subculture, rooting culture and transplanting techniques. The tissue culture formula of the present invention is the best combination for the large-scale multiplication of Mingyue grass, the multiplication coefficient reaches 5-6 times, and the rooting rate and transplanting survival rate are both above 95%. The invention makes up for some deficiencies of the traditional seedling raising technology, improves the reproduction coefficient, shortens the reproduction cycle, and solves the problem of seasonal restrictions. Industrialization requirements for seedling breeding.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture rapid propagation medium and a tissue culture rapid propagation method. Background technique [0002] Angelica keiskei koidzumi (Angelica keiskei koidzumi), also known as golden chicken feather grass, belongs to the genus Asteraceae and is an evergreen upright herbaceous plant with perennial roots. It is native to Singapore. Mingyuecao contains a variety of natural active substances, especially rich in the unique substances chalcone and coumarin (Yin Huidan et al., 2011). Hypoglycemic, hypotensive, antihistamine, anti-cancer effects (Enoki T, et al., 2007). In recent years, Mingyue grass has become a kind of vegetable that people love. It mainly eats stems and leaves. The meat has a fishy smell, and the stems and leaves have a smooth taste and a refreshing taste after being boiled in water. [0003] Mingyue grass is mainly propagated by division o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 杨利平陈乃明王华宇何贵整陈丽文时群杨琼陈乃健陈俊锦梁刚吴红英
Owner 钦州市林业科学研究所