A microfluidic chip device for cell separation
A microfluidic chip and chip technology, applied in the field of microfluidics, can solve the problems of cumbersome operation, error-prone, difficult clinical promotion and application, etc., and achieve the effects of convenient operation, low cost and no manual operation.
Active Publication Date: 2017-10-31
TSINGHUA UNIV +1
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Problems solved by technology
These methods are cumbersome to operate, error-prone, and difficult to apply clinically
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[0030] The present invention will be further described below in conjunction with the accompanying drawings, but the present invention is not limited to the following embodiments.
[0031] Such as figure 1 Shown is the microfluidic chip device for cell separation of the present invention, which includes a centrifugal rotor 1 , a chip structure 2 and a chip cover 3 . In the present invention, if image 3 As shown, two symmetrical chip placement grooves 4 are arranged on the centrifugal rotor 1, and two liquid storage pools 5 are arranged on each side of the chip placement groove 4. The through holes are connected. Such as Figure 4 and Figure 7 As shown, the chip structure 2 in the present invention includes the inner chip 6 and the outer chip 7 of the plexiglass layer material bonded together, and the photoresist dry film 8 arranged between the inner chip 6 and the outer side 7, the inner chip 6 The thickness of the outer chip 7 and the outer chip 7 is 2mm, and the inner ...
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Abstract
The invention discloses a microfluidic chip device for cell separation. It includes a centrifugal rotor, a chip structure and a chip cover; the centrifugal rotor is provided with at least one chip placement groove and at least two liquid storage pools; the liquid storage pools communicate with the through holes provided on the chip placement grooves through channels; the chip structure includes Two integrated chips and filter screens are arranged in the two chips. The inner chip is provided with a fluid inlet connected to the microfluidic channel, and the outer chip is provided with a fluid outlet A and a fluid outlet B. The fluid outlet A is connected to the inner chip. The microfluidic channel on the chip is connected, and the fluid outlet B is connected with the microfluidic channel on the outer chip; the chip structure is fixed in the chip placement groove through the chip cover; the fluid inlet is connected with the liquid storage pool through the through hole, and the fluid outlet A and the fluid outlet B are respectively communicated with the outlets provided on the chip cover. The microfluidic chip device for cell separation of the present invention can use a centrifugation method to separate cells by means of a double-layer chip plus a filter screen, and has the advantages of low price, easy operation, and the like.
Description
technical field [0001] The invention relates to a microfluidic chip device for cell separation, which belongs to the field of microfluidics. Background technique [0002] Fetal nucleated red blood cells (FNRBC) are red blood cells of fetal origin present in the peripheral blood of pregnant women. Compared with other fetal-derived cells present in the peripheral blood of pregnant women, FNRBC has a complete fetal genome, has a variety of specific markers for easy isolation, is easy to identify by morphology or fetal hemoglobin, persists during pregnancy, and has a postpartum The influence of the previous pregnancy can be ruled out, so FNRBC can be better used in prenatal diagnosis to determine the health status of the fetus. Compared with traditional prenatal diagnosis methods such as chorionic villus biopsy, amniocentesis, percutaneous umbilical vascular puncture, etc., the diagnosis using FNRBC only needs to take a small amount of peripheral blood from pregnant women, whic...
Claims
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IPC IPC(8): C12M1/00C12M1/12
Inventor 刘鹏程一淳刘冬辰
Owner TSINGHUA UNIV