A kind of fingerprint detection method of Daji Zhixue tablet and its application
A fingerprint and detection method technology, applied in the field of analysis and detection of traditional Chinese medicine components, can solve problems such as difficult to control the quality of Daji Zhixue Tablets, achieve good precision and repeatability, good stability, and improve quality control levels Effect
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Embodiment 1
[0056] According to the records in the "Ministerial Standards of Traditional Chinese Medicine (Volume 13)" whose standard number is WS3-B-2468-97, Daji Zhixue Tablets were prepared, and the prescription components were 4880g of Dajicao and 120g of dried ginger.
[0057] The specific preparation method is as follows: for the above two flavors, take the large thistle and add water to decoct twice, each time for 1 hour, combine the decoction, filter, and concentrate the filtrate to a relative density of 1.20 (80-85°C), wait for the liquid to cool to At 40-50°C, gradually add ethanol until the alcohol content is 50%, stir well, let it stand for precipitation, take the supernatant and concentrate it under reduced pressure to 1200g for later use. Another dry ginger is crushed into coarse powder, according to the percolation method (Appendix IO) under the item of liquid extract and extract, use 90% ethanol as solvent, percolate after soaking for 24 hours, collect the percolation liqui...
Embodiment 2
[0059] 1. Sample pretreatment
[0060] Preparation of the test solution: Accurately weigh 0.2g of Dajizhixue Tablet powder, place it in a stoppered conical flask, accurately add 1-20ml of ethanol aqueous solution with a volume percentage concentration of 50-100%, weigh it, and seal it tightly. After sealing, ultrasonically extract for 15-60min, let stand to cool, weigh again, make up the weight loss with 50-100% ethanol aqueous solution, and filter with a filter membrane with a pore size of 0.22 μm to obtain the test solution.
[0061] Preparation of the reference substance solution: Accurately weigh 10 mg of the reference substance of rutin, mongoside and salivaryrin leaf glycoside respectively, add 50-100% ethanol aqueous solution to dissolve and shake to a constant volume in a volumetric flask to obtain the reference substance stock solution . The reference stock solution was stored in a refrigerator at 4°C, protected from light. When in use, accurately weigh the referenc...
Embodiment 3
[0075] 1. Sample pretreatment
[0076] Preparation of the test solution: Accurately weigh 0.2g of Dajizhixue Tablet powder, place it in a stoppered conical flask, accurately add 10ml of ethanol aqueous solution with a concentration of 70% by volume, weigh it, seal it tightly and extract it by ultrasonic After 30 minutes, let it stand for cooling, then weigh it again, make up for the weight loss with 70% ethanol aqueous solution, and filter it with a filter membrane with a pore size of 0.22 μm to obtain the test solution.
[0077] Preparation of the reference substance solution: Accurately weigh 10 mg of the reference substances of rutin, mongoside and salivaryrin leaf glycoside respectively, add 70% ethanol aqueous solution to dissolve and shake to a constant volume in a 25ml volumetric flask to obtain the reference substance stock solution. The reference stock solution was stored in a refrigerator at 4°C, protected from light. When in use, accurately weigh the reference subs...
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