A method for improving the identification of astragalus by thin layer chromatography

Abstract

The invention discloses a method raising radix astragali thin-layer chromatographic identification. The method comprises the following steps: firstly, radix astragali is ground into powder, 2-3g of the powder is weighed accurately by a weighing balance, 75ml of trichloromethane-n-butanol is added, heating backflow is carried out for 3h, then filtering is carried out, the filtrate is evaporated to dryness, 8ml-10ml of KOH-methanol saponification liquor is added in the residues, saponification processing is carried out, 8ml-10ml of KOH-methanol saponification liquor is added again, heating backflow is carried out for 0.5h-1h, evaporation to dryness is carried out, the residues are added with water and dissolved in water, ultrasonic extraction is carried out for 30min-40min, ethyl acetate is added, extraction is carried out twice, the final product is employed as a sample, the sample is dipped on the same silica gel G thin layer plate, the same developing solvent is employed, developing is carried out, then the thin layer plate is taken out and dried in air, then the thin layer plate is inspected under light with 365nm of a JL-P10A film viewer or a daylight ultraviolet light lamp, and shooting is carried out finally. The problem is solved that the sharpness of astragaloside in chromatogram is not high and the provided method is an accurate, distinct and rapid method for identifying radix astragali.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for improving identification by TLC of astragalus. Background technique

[0002] This product is the dried root of Astragalus membranaceus or Astragalus membranaceus of the leguminous plant. It is excavated in spring and autumn, and the fibrous roots and root heads are removed, rinsed, cut into thick slices while fresh, and dried. It is called "Astragalus slices". Light brown or light brown, with irregular longitudinal grooves or shrinkage folds. Hard and tough, fibrous and powdery in section, yellowish-white in the skin, light yellow in the wood, with radial texture or fissures. Slight gas, slightly sweet taste, slightly beany smell when chewed.

[0003] The work on the quality standards of traditional Chinese medicine has been in full swing, and scholars of traditional Chinese medicine have put forward some opinions based on the relevant problems in the curre...

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