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A method for improving the identification of astragalus by thin layer chromatography

A technology of thin-layer chromatography and astragalus, applied in the field of improving the identification of astragalus by thin-layer chromatography, can solve the problems of high cost and low chromatographic clarity, and achieve the effect of simple method, obvious effect, and low resolution

Active Publication Date: 2017-04-05
陇西保和堂药业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it needs a neutral alumina column, the cost is high, and its chromatographic clarity is not high

Method used

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  • A method for improving the identification of astragalus by thin layer chromatography
  • A method for improving the identification of astragalus by thin layer chromatography
  • A method for improving the identification of astragalus by thin layer chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) First, grind 10 kinds of astragalus materials from different origins into powder, pass through a 200-mesh sieve, accurately weigh 2 g of the powder with a weighing balance, add 75 ml of chloroform-n-butanol with a volume ratio of 2:1, and heat Reflux, after 3h, filter, then evaporate the filtrate to dryness, add 8mL of KOH-methanol saponification solution with a mass fraction of 20% to the residue, place in a constant temperature water bath at 60°C for 30min; filter, and add 8mL of KOH-methanol saponification solution again , placed in a water bath at 100°C and heated to reflux for 1 hour, evaporated to dryness, added 30ml of water to dissolve the residue, and moved it to a separatory funnel; then added 2mL of methanol to fully dissolve the residue of Astragalus membranaceus, then extracted by ultrasonic for 30min, the ultrasonic power was 50W, and the ultrasonic temperature was 4°C , after extracting twice, add ethyl acetate to extract twice, each time 30mL, evapora...

Embodiment 2

[0028] (1) First, grind 10 kinds of astragalus materials from different origins into powder, pass through a 200-mesh sieve, accurately weigh 2 g of the powder with a weighing balance, add 75 ml of chloroform-n-butanol with a volume ratio of 2:1, and heat Reflux, after 3h, filter, then evaporate the filtrate to dryness, add 8mL of KOH-methanol saponification solution with a mass fraction of 20% to the residue, place in a constant temperature water bath at 60°C for 30min; filter, and add 8mL of KOH-methanol saponification solution again , placed in a water bath at 100°C and heated to reflux for 1 hour, evaporated to dryness, added 30ml of water to dissolve the residue, and moved it to a separatory funnel; then added 2mL of methanol to fully dissolve the residue of Astragalus membranaceus, then extracted by ultrasonic for 30min, the ultrasonic power was 50W, and the ultrasonic temperature was 4°C , after extracting twice, add ethyl acetate to extract twice, each time 30mL, evapora...

Embodiment 3

[0031] (1) First, grind 10 kinds of astragalus materials from different origins into powder, pass through a 200-mesh sieve, accurately weigh 3g of the powder with a weighing balance, add 75ml of chloroform-n-butanol with a volume ratio of 2:1, and heat Reflux, after 3h, filter, then evaporate the filtrate to dryness, add 10mL of KOH-methanol saponification solution with a mass fraction of 20% to the residue, place in a constant temperature water bath at 60°C for 30min; filter, and add 10mL of KOH-methanol saponification solution again , placed in a water bath at 100°C and heated to reflux for 1 hour, evaporated to dryness, added 30ml of water to dissolve the residue, and moved it to a separatory funnel; then added 2mL of methanol to fully dissolve the residue of Astragalus membranaceus, then extracted by ultrasonic for 30min, the ultrasonic power was 50W, and the ultrasonic temperature was 4°C , after extracting twice, add ethyl acetate to extract twice, each time 30mL, evapora...

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Abstract

The invention discloses a method raising radix astragali thin-layer chromatographic identification. The method comprises the following steps: firstly, radix astragali is ground into powder, 2-3g of the powder is weighed accurately by a weighing balance, 75ml of trichloromethane-n-butanol is added, heating backflow is carried out for 3h, then filtering is carried out, the filtrate is evaporated to dryness, 8ml-10ml of KOH-methanol saponification liquor is added in the residues, saponification processing is carried out, 8ml-10ml of KOH-methanol saponification liquor is added again, heating backflow is carried out for 0.5h-1h, evaporation to dryness is carried out, the residues are added with water and dissolved in water, ultrasonic extraction is carried out for 30min-40min, ethyl acetate is added, extraction is carried out twice, the final product is employed as a sample, the sample is dipped on the same silica gel G thin layer plate, the same developing solvent is employed, developing is carried out, then the thin layer plate is taken out and dried in air, then the thin layer plate is inspected under light with 365nm of a JL-P10A film viewer or a daylight ultraviolet light lamp, and shooting is carried out finally. The problem is solved that the sharpness of astragaloside in chromatogram is not high and the provided method is an accurate, distinct and rapid method for identifying radix astragali.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for improving identification by TLC of astragalus. Background technique [0002] This product is the dried root of Astragalus membranaceus or Astragalus membranaceus of the leguminous plant. It is excavated in spring and autumn, and the fibrous roots and root heads are removed, rinsed, cut into thick slices while fresh, and dried. It is called "Astragalus slices". Light brown or light brown, with irregular longitudinal grooves or shrinkage folds. Hard and tough, fibrous and powdery in section, yellowish-white in the skin, light yellow in the wood, with radial texture or fissures. Slight gas, slightly sweet taste, slightly beany smell when chewed. [0003] The work on the quality standards of traditional Chinese medicine has been in full swing, and scholars of traditional Chinese medicine have put forward some opinions based on the relevant problems in the curre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 马中森安莹娟赵洋洋董立定李慧林潘丽娟常文军李斌梁爱军李宏强
Owner 陇西保和堂药业有限责任公司
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