The invention discloses a method raising radix astragali thin-layer chromatographic identification. The method comprises the following steps: firstly, radix astragali is ground into
powder, 2-3g of the
powder is weighed accurately by a weighing balance, 75ml of trichloromethane-n-
butanol is added, heating
backflow is carried out for 3h, then filtering is carried out, the filtrate is evaporated to
dryness, 8ml-10ml of KOH-
methanol saponification liquor is added in the residues,
saponification processing is carried out, 8ml-10ml of KOH-
methanol saponification liquor is added again, heating
backflow is carried out for 0.5h-1h,
evaporation to
dryness is carried out, the residues are added with water and dissolved in water, ultrasonic extraction is carried out for 30min-40min,
ethyl acetate is added, extraction is carried out twice, the final product is employed as a sample, the sample is dipped on the same
silica gel G
thin layer plate, the same developing
solvent is employed, developing is carried out, then the
thin layer plate is taken out and dried in air, then the
thin layer plate is inspected under light with 365nm of a JL-P10A film viewer or a
daylight ultraviolet light lamp, and shooting is carried out finally. The problem is solved that the sharpness of
astragaloside in chromatogram is not high and the provided method is an accurate, distinct and rapid method for identifying radix astragali.